Protocol Protocol for MRI-guided virus injection in macaque deep brain regions

被引：0

作者：

Yu, Panke ^{[1
,2
,4
]}

Zhang, Zhiting ^{[1
,2
]}

Wang, Yuyin ^{[1
,2
]}

Dai, Ji ^{[1
,2
,3
,4
]}

机构：

[1] Chinese Acad Sci, Shenzhen Hong Kong Inst Brain Sci, Shenzhen Inst Adv Technol, Shenzhen Technol Res Ctr Primate Translat Med, Shenzhen 518055, Peoples R China

[2] Chinese Acad Sci, Brain Cognit & Brain Dis Inst, Shenzhen Inst Adv Technol, CAS Key Lab Brain Connectome & Manipulat, Shenzhen 518055, Peoples R China

[3] Chinese Acad Sci, Shenzhen Inst Adv Technol, Guangdong Prov Key Lab Brain Connectome & Behav, Shenzhen 518055, Peoples R China

[4] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Behavior; Cognitive Neuroscience;

D O I：

10.1016/j.xpro.2023.102768

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Effective delivery of viruses into required brain regions is critical to the success of optogenetic or chemogenetic experiments. However, in monkeys, due to the large size and heterogeneity of their brain, precise injections in deep brain re-gions have been challenging. Here, we present a protocol for virus injection in monkey deep brain regions under the guidance of MRI. We describe the steps for installing the guiding grid, MRI scanning, MRI-based localization, and virus in-jection.For complete details on the use and execution of this protocol, please refer to Chen et al. (2023).1

引用

页数：14

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