Extraction DNA from Activated Sludge-Comparing with Soil Sample

被引：0
|
作者：
谢冰
奚旦立
陈季华
机构：
[1] Donghua University
[2] School of Environmental Science and Engineering
[3] Shanghai
来源：
Journal of DongHua University | 2003年 / 01期
关键词：
DNA extraction; PCR (polymerase chain reaction); activated sludge; soil; heavy metal;
D O I：
10.19884/j.1672-5220.2003.01.029
中图分类号：
Q523 [脱氧核糖核酸（DNA）];
学科分类号：
摘要：
DNA directly extraction from activated sludge andsoil sample with enzyme lyses methods wasinvestigated in this paper. DNA yield from activatedsludge was 3. 0 mg/g. MLSS, and 28. 2-43. 8μg/g soilrespectively. The resulting DNA is suitable for PCR.By studied methods, higher quality and quantity of
引用
收藏
页码：127 / 130
页数：4
相关论文
共 50 条
  • [41] METHODOLOGY FOR COMPARING POWDERED ACTIVATED CARBONS FOR ACTIVATED-SLUDGE ADDITION
    FLYNN, BP
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1974, : 33 - 33
  • [42] Extraction of activated sludge bacteria exopolymers by ultrasonication
    Matias, VRF
    Cammarota, MC
    Sant'Anna, GL
    BIOTECHNOLOGY LETTERS, 2003, 25 (16) : 1351 - 1356
  • [43] Extraction of activated sludge bacteria exopolymers by ultrasonication
    V.R.F. Matias
    M.C. Cammarota
    G.L. Sant'Anna
    Biotechnology Letters, 2003, 25 : 1351 - 1356
  • [44] Alkaline extraction of wastewater activated sludge biosolids
    Becerra, Flor Y. Garcia
    Acosta, Edgar J.
    Allen, D. Grant
    BIORESOURCE TECHNOLOGY, 2010, 101 (18) : 6972 - 6980
  • [45] Bacterial community analysis of activated sludge: an evaluation of four commonly used DNA extraction methods
    Louise Vanysacker
    Steven A. J. Declerck
    Bart Hellemans
    Luc De Meester
    Ivo Vankelecom
    Priscilla Declerck
    Applied Microbiology and Biotechnology, 2010, 88 : 299 - 307
  • [46] Back to Basics - The Influence of DNA Extraction and Primer Choice on Phylogenetic Analysis of Activated Sludge Communities
    Albertsen, Mads
    Karst, Soren M.
    Ziegler, Anja S.
    Kirkegaard, Rasmus H.
    Nielsen, Per H.
    PLOS ONE, 2015, 10 (07):
  • [47] Bacterial community analysis of activated sludge: an evaluation of four commonly used DNA extraction methods
    Vanysacker, Louise
    Declerck, Steven A. J.
    Hellemans, Bart
    De Meester, Luc
    Vankelecom, Ivo
    Declerck, Priscilla
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2010, 88 (01) : 299 - 307
  • [48] The effect of soil sample size, for practical DNA extraction, on soil microbial diversity in different taxonomic ranks
    Morita, Hiroki
    Akao, Satoshi
    PLOS ONE, 2021, 16 (11):
  • [49] Effects of Different Methods of DNA Extraction for Activated Sludge on the Subsequent Analysis of Bacterial Community Profiles
    Sun, Lianpeng
    Ouyang, Xiong
    Tang, Yueheng
    Yang, Ying
    Luo, Ying
    WATER ENVIRONMENT RESEARCH, 2012, 84 (02) : 108 - 114
  • [50] Activated carbon from activated sludge
    Bosch, H.
    Kleerebezem, G.J.
    Mars, P.
    1600, (48):
12345