Enrichment, Antioxidant and Enzyme Inhibition Activities of Flavonoids from Artemisia Selengensis Turcz.

Macroporous resin was used to enrich flavonoids in the ethyl acetate extract of Artemisia Selengensis Turcz. Based on a single factor experiment, the enrichment process was optimized using the response surface method. The optimal parameters of the enrichment process were a sample concentration of 0.3 mg/mL, a loading rate of 1 mL/min, an elution flow rate of 2 mL/min, and a total flavonoid content of 155.38 +/- 0.97 mg/g. The flavonoids enriched by AB-8 macroporous resin demonstrated significant scavenging activities against DPPH, ABTS+, and hydroxyl free radicals, and also exhibited certain inhibitory effects on alpha-amylase and alpha-glucosidase. Among them, the scavenging ability of the flavonoids enriched by AB-8 macroporous resin on hydroxyl free radical (IC50=30.31 +/- 1.92 mu g/mL) was the closest to Vc, and the inhibitory effect on alpha-glucosidase (IC50=16.19 +/- 1.35 mu g/mL) was the best. These findings confirmed the potential of Artemisia Selengensis Turcz. was a natural antioxidant and hypoglycemic drug.