Quantification of lysosomal labile Zn2+and monitoring of Zn2+efflux using a small-molecule-protein hybrid fluorescent probe

被引：0

作者：

Du, Yuyin ^{[1
]}

Kowada, Toshiyuki ^{[2
,3
,4
]}

Sung, Eunhye ^{[4
]}

Liu, Rong ^{[2
]}

Soloviev, Andrei ^{[3
]}

Matsui, Toshitaka ^{[2
,3
,4
]}

Mizukami, Shin ^{[2
,3
,4
]}

机构：

[1] Tohoku Univ, Fac Sci, Dept Chem, 6-3 Aramaki Aza Aoba,Aoba Ku, Sendai, Miyagi 9808578, Japan

[2] Tohoku Univ, Inst Multidisciplinary Res Adv Mat, 2-1-1 Katahira,Aoba Ku, Sendai, Miyagi 9808577, Japan

[3] Tohoku Univ, Grad Sch Life Sci, 2-1-1 Katahira,Aoba Ku, Sendai, Miyagi 9808577, Japan

[4] Tohoku Univ, Grad Sch Sci, Dept Chem, 6-3 Aramaki Aza Aoba,Aoba Ku, Sendai, Miyagi 9808578, Japan

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 2025年 / 264卷

关键词：

Zinc; Lysosome; Fluorescence imaging; TRPMLs; HaloTag; Protein labeling; ZINC; RELEASE; INDICATOR; SENSOR;

D O I：

10.1016/j.jinorgbio.2024.112811

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lysosomal labile Zn2+ levels have been unclear. By targeting a small-molecule fluorescent Zn2+ probe, ZnDA-3H, to lysosomes via VAMP7-Halo, the lysosomal labile Zn2+ concentration was determined to be 1.9 nM in HeLa cells. Furthermore, ZnDA-3H enabled direct visualization of the Zn2+ efflux from the lysosomes to cytosol upon TRPMLs activation.

引用

页数：5

共 39 条

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