Quantification of lysosomal labile Zn2+and monitoring of Zn2+efflux using a small-molecule-protein hybrid fluorescent probe

被引：0
|
作者：
Du, Yuyin [1 ]
Kowada, Toshiyuki [2 ,3 ,4 ]
Sung, Eunhye [4 ]
Liu, Rong [2 ]
Soloviev, Andrei [3 ]
Matsui, Toshitaka [2 ,3 ,4 ]
Mizukami, Shin [2 ,3 ,4 ]
机构：
[1] Tohoku Univ, Fac Sci, Dept Chem, 6-3 Aramaki Aza Aoba,Aoba Ku, Sendai, Miyagi 9808578, Japan
[2] Tohoku Univ, Inst Multidisciplinary Res Adv Mat, 2-1-1 Katahira,Aoba Ku, Sendai, Miyagi 9808577, Japan
[3] Tohoku Univ, Grad Sch Life Sci, 2-1-1 Katahira,Aoba Ku, Sendai, Miyagi 9808577, Japan
[4] Tohoku Univ, Grad Sch Sci, Dept Chem, 6-3 Aramaki Aza Aoba,Aoba Ku, Sendai, Miyagi 9808578, Japan
来源：
JOURNAL OF INORGANIC BIOCHEMISTRY | 2025年 / 264卷
关键词：
Zinc; Lysosome; Fluorescence imaging; TRPMLs; HaloTag; Protein labeling; ZINC; RELEASE; INDICATOR; SENSOR;
D O I：
10.1016/j.jinorgbio.2024.112811
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Lysosomal labile Zn2+ levels have been unclear. By targeting a small-molecule fluorescent Zn2+ probe, ZnDA-3H, to lysosomes via VAMP7-Halo, the lysosomal labile Zn2+ concentration was determined to be 1.9 nM in HeLa cells. Furthermore, ZnDA-3H enabled direct visualization of the Zn2+ efflux from the lysosomes to cytosol upon TRPMLs activation.
引用
收藏
页数：5
相关论文
共 39 条
  • [21] Recent Advances in Organic Small-Molecule Fluorescent Probes for the Detection of Zinc Ions (Zn2+)
    Yang, Junjie
    Tang, Liting
    Li, Lin
    Wu, Xiongzhi
    Yan, Liqiang
    JOURNAL OF FLUORESCENCE, 2024,
  • [22] Direct Comparison of a Genetically Encoded Sensor and Small Molecule Indicator: Implications for Quantification of Cytosolic Zn2+
    Qin, Yan
    Miranda, Jose G.
    Stoddard, Caitlin I.
    Dean, Kevin M.
    Galati, Domenico F.
    Palmer, Amy E.
    ACS CHEMICAL BIOLOGY, 2013, 8 (11) : 2366 - 2371
  • [23] CORRELATION OF APOPTOSIS WITH CHANGE IN INTRACELLULAR LABILE ZN(II) USING ZINQUIN [(2-METHYL-8-P-TOLUENESULPHONAMIDO-6-QUINOLYLOXY)ACETIC ACID], A NEW SPECIFIC FLUORESCENT-PROBE FOR ZN(II)
    ZALEWSKI, PD
    FORBES, IJ
    BETTS, WH
    BIOCHEMICAL JOURNAL, 1993, 296 : 403 - 408
  • [24] Detection and Quantification of Tightly Bound Zn2+ in Blood Serum Using a Photocaged Chelator and a DNAzyme Fluorescent Sensor
    Xing, Shige
    Lin, Yao
    Cai, Liangyuan
    Basa, Prem N.
    Shigemoto, Austin K.
    Zheng, Chengbin
    Zhang, Feng
    Burdette, Shawn C.
    Lu, Yi
    ANALYTICAL CHEMISTRY, 2021, 93 (14) : 5856 - 5861
  • [25] Real Time Imaging and Dynamics of Hippocampal Zn2+ under Epileptic Condition Using a Ratiometric Fluorescent Probe
    Hema Santhakumar
    Resmi. V. Nair
    Divya Susan Philips
    Sachin J. Shenoy
    Anoopkumar Thekkuveettil
    Ayyappanpillai Ajayaghosh
    Ramapurath. S. Jayasree
    Scientific Reports, 8
  • [26] Real Time Imaging and Dynamics of Hippocampal Zn2+ under Epileptic Condition Using a Ratiometric Fluorescent Probe
    Santhakumar, Hema
    Nair, Resmi. V.
    Philips, Divya Susan
    Shenoy, Sachin J.
    Thekkuveettil, Anoopkumar
    Ajayaghosh, Ayyappanpillai
    Jayasree, Ramapurath S.
    SCIENTIFIC REPORTS, 2018, 8
  • [27] A benzothiazole-based fluorescent probe for efficient detection and discrimination of Zn2+ and Cd2+, using cysteine as an auxiliary reagent
    Li, Jia
    Chen, Yahui
    Chen, Tiantian
    Qiang, Jian
    Zhang, Zhijie
    Wei, Tingwen
    Zhang, Wei
    Wang, Fang
    Chen, Xiaoqiang
    SENSORS AND ACTUATORS B-CHEMICAL, 2018, 268 : 446 - 455
  • [28] Effective Determination of Zn2+, Mn2+, and Cu2+ Simultaneously By Using Dual-Emissive Carbon Dots as Colorimetric Fluorescent Probe
    Yu, Liying
    Ren, Guojuan
    Tang, Mingyu
    Zhu, Baoya
    Chai, Fang
    Li, Gang
    Xu, Dandan
    EUROPEAN JOURNAL OF INORGANIC CHEMISTRY, 2018, (29) : 3418 - 3426
  • [29] A naphthalene-thiophene hybrid molecule as a fluorescent AND logic gate with Zn2+ and OAc- ions as inputs: cell imaging and computational studies
    Karak, Debasis
    Das, Sudipta
    Lohar, Sisir
    Banerjee, Arnab
    Sahana, Animesh
    Hauli, Ipsit
    Mukhopadhyay, Subhra Kanti
    Safin, Damir A.
    Babashkina, Maria G.
    Bolte, Michael
    Garcia, Yann
    Das, Debasis
    DALTON TRANSACTIONS, 2013, 42 (19) : 6708 - 6715
  • [30] Reaction-Based "Off On" Fluorescent Probe Enabling Detection of Endogenous Labile Fe2+ and Imaging of Zn2+-induced Fe2+ Flux in Living Cells and Elevated Fe2+ in Ischemic Stroke
    Xuan, Weimin
    Pan, Rong
    Wei, Yongyi
    Cao, Yanting
    Li, Huanqiu
    Liang, Fu-Sen
    Liu, Ke-Jian
    Wang, Wei
    BIOCONJUGATE CHEMISTRY, 2016, 27 (02) : 302 - 308
1234