Streamlined Nucleus Isolation Protocol for Cryopreserved Mouse Lung Captures Non-immune Cell Types by Single-nucleus RNA Sequencing

被引:0
|
作者
Tolman, N. J. [1 ]
Tabary, M. [1 ]
Sharma, L. [1 ]
Dela Cruz, C. [1 ]
Alder, J. K. [1 ]
Lee, J. [2 ]
Chen, K. [1 ]
Bain, W. [1 ]
机构
[1] Univ Pittsburgh, Div Pulm Allergy Crit Care & Sleep Med, Pittsburgh, PA USA
[2] Washington Univ, Div Pulm & Crit Care Med, St Louis, MO 63110 USA
关键词
D O I
暂无
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
A3315
引用
收藏
页数:2
相关论文
共 50 条
  • [41] Dissociation protocols used for sarcoma tissues bias the transcriptome observed in single-cell and single-nucleus RNA sequencing
    Danh D. Truong
    Salah-Eddine Lamhamedi-Cherradi
    Robert W. Porter
    Sandhya Krishnan
    Jyothishmathi Swaminathan
    Amber Gibson
    Alexander J. Lazar
    J. Andrew Livingston
    Vidya Gopalakrishnan
    Nancy Gordon
    Najat C. Daw
    Nicholas E. Navin
    Richard Gorlick
    Joseph A. Ludwig
    BMC Cancer, 23
  • [42] Dissociation protocols used for sarcoma tissues bias the transcriptome observed in single-cell and single-nucleus RNA sequencing
    Truong, Danh D.
    Lamhamedi-Cherradi, Salah-Eddine
    Porter, Robert W.
    Krishnan, Sandhya
    Swaminathan, Jyothishmathi
    Gibson, Amber
    Lazar, Alexander J.
    Livingston, J. Andrew
    Gopalakrishnan, Vidya
    Gordon, Nancy
    Daw, Najat C.
    Navin, Nicholas E.
    Gorlick, Richard
    Ludwig, Joseph A.
    BMC CANCER, 2023, 23 (01)
  • [43] Dynamic Transcriptional Responses to Injury of Regenerative and Non-regenerative Cardiomyocytes Revealed by Single-Nucleus RNA Sequencing
    Cui, Miao
    Wang, Zhaoning
    Chen, Kenian
    Shah, Akansha M.
    Tan, Wei
    Duan, Lauren
    Sanchez-Ortiz, Efrain
    Li, Hui
    Xu, Lin
    Liu, Ning
    Bassel-Duby, Rhonda
    Olson, Eric N.
    DEVELOPMENTAL CELL, 2020, 53 (01) : 102 - +
  • [44] Investigating immune and non-immune cell interactions in head and neck tumors by single-cell RNA sequencing
    Cornelius H. L. Kürten
    Aditi Kulkarni
    Anthony R. Cillo
    Patricia M. Santos
    Anna K. Roble
    Sayali Onkar
    Carly Reeder
    Stephan Lang
    Xueer Chen
    Umamaheswar Duvvuri
    Seungwon Kim
    Angen Liu
    Tracy Tabib
    Robert Lafyatis
    Jian Feng
    Shou-Jiang Gao
    Tullia C. Bruno
    Dario A. A. Vignali
    Xinghua Lu
    Riyue Bao
    Lazar Vujanovic
    Robert L. Ferris
    Nature Communications, 12
  • [45] Investigating immune and non-immune cell interactions in head and neck tumors by single-cell RNA sequencing
    Kuerten, Cornelius H. L.
    Kulkarni, Aditi
    Cillo, Anthony R.
    Santos, Patricia M.
    Roble, Anna K.
    Onkar, Sayali
    Reeder, Carly
    Lang, Stephan
    Chen, Xueer
    Duvvuri, Umamaheswar
    Kim, Seungwon
    Liu, Angen
    Tabib, Tracy
    Lafyatis, Robert
    Feng, Jian
    Gao, Shou-Jiang
    Bruno, Tullia C.
    Vignali, Dario A. A.
    Lu, Xinghua
    Bao, Riyue
    Vujanovic, Lazar
    Ferris, Robert L.
    NATURE COMMUNICATIONS, 2021, 12 (01)
  • [46] Bulk and single-nucleus RNA sequencing highlight immune pathways induced in individuals during an Ixodes scapularis tick bite
    Tang, Xiaotian
    Lynn, Geoffrey E.
    Cui, Yingjun
    Cerny, Jiri
    Arora, Gunjan
    Tomayko, Mary M.
    Craft, Joseph
    Fikrig, Erol
    INFECTION AND IMMUNITY, 2023, 91 (11)
  • [47] Single-Nucleus RNA Sequencing Reveals Region-Specific Cell Composition and Transcriptional Variations in Cardiac Sarcoidosis
    Neyazi, Meraj
    Kim, Yuri
    Brown, Kemar
    Barish, Syndi
    Gorham, Joshua
    Layton, Olivia
    Viveiros, Anissa
    DeLaughter, Daniel
    Beyer, Martin
    Strohmenger, Viktoria
    Reichart, Daniel
    Bowles, Dawn
    Glass, Carolyn
    Oudit, Gavin
    Seidman, Jonathan
    Seidman, Christine
    CIRCULATION, 2024, 150
  • [48] Defining Specific Cell States of MPTP-Induced Parkinson's Disease by Single-Nucleus RNA Sequencing
    Guo, Yunxia
    Ma, Junjie
    Huang, Hao
    Xu, Jitao
    Jiang, Chao
    Ye, Kaiqiang
    Chang, Ning
    Ge, Qinyu
    Wang, Guangzhong
    Zhao, Xiangwei
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (18)
  • [49] Spatial transcriptomics combined with single-nucleus RNA sequencing reveals glial cell heterogeneity in the human spinal cord
    Yali Chen
    Yiyong Wei
    Jin Liu
    Tao Zhu
    Cheng Zhou
    Donghang Zhang
    Neural Regeneration Research, 2025, 20 (11) : 3302 - 3316
  • [50] Spatial transcriptomics combined with single-nucleus RNA sequencing reveals glial cell heterogeneity in the human spinal cord
    Chen, Yali
    Wei, Yiyong
    Liu, Jin
    Zhu, Tao
    Zhou, Cheng
    Zhang, Donghang
    NEURAL REGENERATION RESEARCH, 2025, 20 (11) : 3302 - 3316