Streamlined Nucleus Isolation Protocol for Cryopreserved Mouse Lung Captures Non-immune Cell Types by Single-nucleus RNA Sequencing

被引：0
|
作者：
Tolman, N. J. [1 ]
Tabary, M. [1 ]
Sharma, L. [1 ]
Dela Cruz, C. [1 ]
Alder, J. K. [1 ]
Lee, J. [2 ]
Chen, K. [1 ]
Bain, W. [1 ]
机构：
[1] Univ Pittsburgh, Div Pulm Allergy Crit Care & Sleep Med, Pittsburgh, PA USA
[2] Washington Univ, Div Pulm & Crit Care Med, St Louis, MO 63110 USA
来源：
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE | 2024年 / 209卷
关键词：
D O I：
暂无
中图分类号：
R4 [临床医学];
学科分类号：
1002 ; 100602 ;
摘要：
A3315
引用
收藏
页数：2
相关论文
共 50 条
  • [21] Complementary Roles for Single-Nucleus and Single-Cell RNA Sequencing in Kidney Disease Research
    O'Sullivan, Eoin D.
    Mylonas, Katie J.
    Hughes, Jeremy
    Ferenbach, David A.
    JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2019, 30 (04): : 712 - 713
  • [22] Single-Nucleus and In Situ RNA-Sequencing Reveal Cell Topographies in the Human Pancreas
    Tosti, Luca
    Hang, Yan
    Debnath, Olivia
    Tiesmeyer, Sebastian
    Trefzer, Timo
    Steiger, Katja
    Ten, Foo Wei
    Lukassen, Soeren
    Ballke, Simone
    Kuehl, Anja A.
    Spieckermann, Simone
    Bottino, Rita
    Ishaque, Naveed
    Weichert, Wilko
    Kim, Seung K.
    Eils, Roland
    Conrad, Christian
    GASTROENTEROLOGY, 2021, 160 (04) : 1330 - +
  • [23] The neuronal and glial cell diversity in the celiac ganglion revealed by single-nucleus RNA sequencing
    Kanda, Hirosato
    Yamanaka, Hiroki
    Dai, Yi
    Noguchi, Koichi
    SCIENTIFIC REPORTS, 2025, 15 (01):
  • [24] Single-nucleus RNA sequencing of mouse auditory cortex reveals critical period triggers and brakes
    Kalish, Brian T.
    Barkat, Tania R.
    Diel, Erin E.
    Zhang, Elizabeth J.
    Greenberg, Michael E.
    Hensch, Takao K.
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2020, 117 (21) : 11744 - 11752
  • [25] Updated skin transcriptomic atlas depicted by reciprocal contribution of single-nucleus RNA sequencing and single-cell RNA sequencing
    Zhu, Ronghui
    Pan, Xiaoyu
    Wang, Shangshang
    Qiu, Zhuoqiong
    Gu, Chaoying
    Yao, Xu
    Li, Wei
    JOURNAL OF DERMATOLOGICAL SCIENCE, 2023, 111 (02) : 22 - 31
  • [26] Updated skin transcriptomic atlas depicted by reciprocal contribution of single-nucleus RNA sequencing and single-cell RNA sequencing
    Zhu, R.
    Pan, X.
    Wang, S.
    Qiong, Q.
    Gu, C.
    Yao, X.
    Li, W.
    JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2023, 143 (05) : S231 - S231
  • [27] Advantages of Single-Nucleus over Single-Cell RNA Sequencing of Adult Kidney: Rare Cell Types and Novel Cell States Revealed in Fibrosis
    Wu, Haojia
    Kirita, Yuhei
    Donnelly, Erinn L.
    Humphreys, Benjamin D.
    JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2019, 30 (01): : 23 - 32
  • [28] Isolation of Nuclei from Human Snap-frozen Liver Tissue for Single-nucleus RNA Sequencing
    Alvarez, Marcus
    Benhammou, Jihane N.
    Rao, Shuyun
    Mishra, Lopa
    Pisegna, Joseph R.
    Pajukanta, Paivi
    BIO-PROTOCOL, 2023, 13 (03):
  • [29] Current Methodological Challenges of Single-Cell and Single-Nucleus RNA-Sequencing in Glomerular Diseases
    Deleersnijder, Dries
    Callemeyn, Jasper
    Arijs, Ingrid
    Naesens, Maarten
    Van Craenenbroeck, Amaryllis H.
    Lambrechts, Diether
    Sprangers, Ben
    JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2021, 32 (08): : 1838 - 1852
  • [30] Author Correction: Systematic comparison of single-cell and single-nucleus RNA-sequencing methods
    Jiarui Ding
    Xian Adiconis
    Sean K. Simmons
    Monika S. Kowalczyk
    Cynthia C. Hession
    Nemanja D. Marjanovic
    Travis K. Hughes
    Marc H. Wadsworth
    Tyler Burks
    Lan T. Nguyen
    John Y. H. Kwon
    Boaz Barak
    William Ge
    Amanda J. Kedaigle
    Shaina Carroll
    Shuqiang Li
    Nir Hacohen
    Orit Rozenblatt-Rosen
    Alex K. Shalek
    Alexandra-Chloé Villani
    Aviv Regev
    Joshua Z. Levin
    Nature Biotechnology, 2020, 38 : 756 - 756
12345