Protocol for efficient generation of human artery and vein endothelial cells from pluripotent stem cells

被引：1

作者：

Loh, Kyle M. ^{[1
,2
]}

Zheng, Sherry Li ^{[1
,2
]}

Liu, Kevin J. ^{[1
]}

Yin, Qingqing ^{[1
,2
]}

Amir-Ugokwe, Zhainib A. ^{[1
,3
]}

Jha, Sawan K. ^{[3
]}

Qi, Yue ^{[4
]}

Wazny, Vanessa K. ^{[5
]}

Nguyen, Alana T. ^{[1
,8
]}

Chen, Angela ^{[1
,9
]}

Njunkeng, Faith-Masong ^{[6
]}

Cheung, Christine ^{[5
,7
]}

Spiekerkoetter, Edda

Red-Horse, Kristy ^{[1
,3
]}

Ang, Lay Teng ^{[1
,6
]}

机构：

[1] Stanford Univ, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Dev Biol, Stanford, CA 94305 USA

[3] Stanford Univ, Howard Hughes Med Inst, Dept Biol, Stanford, CA 94305 USA

[4] Stanford Univ, Vera Moulton Wall Ctr Pulm Vasc Dis, Dept Med, Div Pulm Allergy & Crit Care Med, Stanford, CA 94305 USA

[5] Nanyang Technol Univ, Lee Kong Chian Sch Med, Singapore 308232, Singapore

[6] Stanford Univ, Dept Urol, Stanford, CA 94305 USA

[7] ASTAR, Inst Mol & Cell Biol, Singapore 138673, Singapore

[8] Univ Calif Davis, Sch Vet Med, Davis, CA 95616 USA

[9] Orca Bio, Menlo Pk, CA 94025 USA

来源：

STAR PROTOCOLS | 2025年 / 6卷 / 01期

关键词：

BONE MORPHOGENETIC PROTEIN-4; MESODERM FORMATION; PRIMITIVE STREAK; DIFFERENTIATION; INHIBITORS; SPECIFICATION; ACTIVATION; KINASE; VEGF; DISSOCIATION;

D O I：

10.1016/j.xpro.2024.103494

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Blood vessels permeate all organs and execute myriad roles in health and disease. Here, we present a protocol to efficiently generate human artery and vein endothelial cells (ECs) from pluripotent stem cells within 3-4 days of differentiation. We delineate how to seed human pluripotent stem cells and sequentially differentiate them into primitive streak, lateral mesoderm, and either artery or vein ECs. We differentiate stem cells in defined, serum-free culture media in monolayers, without feeder cells or genetic manipulations. For complete details on the use and execution of this protocol, please refer to Ang et al.

引用

页数：38

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