Protocol for efficient generation of human artery and vein endothelial cells from pluripotent stem cells

被引：1

作者：

Loh, Kyle M. ^{[1
,2
]}

Zheng, Sherry Li ^{[1
,2
]}

Liu, Kevin J. ^{[1
]}

Yin, Qingqing ^{[1
,2
]}

Amir-Ugokwe, Zhainib A. ^{[1
,3
]}

Jha, Sawan K. ^{[3
]}

Qi, Yue ^{[4
]}

Wazny, Vanessa K. ^{[5
]}

Nguyen, Alana T. ^{[1
,8
]}

Chen, Angela ^{[1
,9
]}

Njunkeng, Faith-Masong ^{[6
]}

Cheung, Christine ^{[5
,7
]}

Spiekerkoetter, Edda

Red-Horse, Kristy ^{[1
,3
]}

Ang, Lay Teng ^{[1
,6
]}

机构：

[1] Stanford Univ, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Dev Biol, Stanford, CA 94305 USA

[3] Stanford Univ, Howard Hughes Med Inst, Dept Biol, Stanford, CA 94305 USA

[4] Stanford Univ, Vera Moulton Wall Ctr Pulm Vasc Dis, Dept Med, Div Pulm Allergy & Crit Care Med, Stanford, CA 94305 USA

[5] Nanyang Technol Univ, Lee Kong Chian Sch Med, Singapore 308232, Singapore

[6] Stanford Univ, Dept Urol, Stanford, CA 94305 USA

[7] ASTAR, Inst Mol & Cell Biol, Singapore 138673, Singapore

[8] Univ Calif Davis, Sch Vet Med, Davis, CA 95616 USA

[9] Orca Bio, Menlo Pk, CA 94025 USA

来源：

STAR PROTOCOLS | 2025年 / 6卷 / 01期

关键词：

BONE MORPHOGENETIC PROTEIN-4; MESODERM FORMATION; PRIMITIVE STREAK; DIFFERENTIATION; INHIBITORS; SPECIFICATION; ACTIVATION; KINASE; VEGF; DISSOCIATION;

D O I：

10.1016/j.xpro.2024.103494

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Blood vessels permeate all organs and execute myriad roles in health and disease. Here, we present a protocol to efficiently generate human artery and vein endothelial cells (ECs) from pluripotent stem cells within 3-4 days of differentiation. We delineate how to seed human pluripotent stem cells and sequentially differentiate them into primitive streak, lateral mesoderm, and either artery or vein ECs. We differentiate stem cells in defined, serum-free culture media in monolayers, without feeder cells or genetic manipulations. For complete details on the use and execution of this protocol, please refer to Ang et al.

引用

页数：38

共 50 条

[31] Generation, expansion and functional analysis of endothelial cells and pericytes derived from human pluripotent stem cells
Valeria V Orlova
Francijna E van den Hil
Sandra Petrus-Reurer
Yvette Drabsch
Peter ten Dijke
Christine L Mummery
Nature Protocols, 2014, 9 : 1514 - 1531
[32] A novel efficient strategy to generate liver sinusoidal endothelial cells from human pluripotent stem cells
Tian, Shang-Ping
Ge, Jian-Yun
Song, Yu-Mu
Yu, Xiao-Qing
Chen, Wen-Hao
Chen, Yu-Ying
Ye, Di
Zheng, Yun-Wen
SCIENTIFIC REPORTS, 2024, 14 (01):
[33] Efficient generation of endothelial cells from induced pluripotent stem cells derived from a patient with peripheral arterial disease
Bootsakorn Boonkaew
Siriwal Suwanpitak
Kovit Pattanapanyasat
Nuttawut Sermsathanasawadi
Methichit Wattanapanitch
Cell and Tissue Research, 2022, 388 : 89 - 104
[34] Efficient generation of endothelial cells from induced pluripotent stem cells derived from a patient with peripheral arterial disease
Boonkaew, Bootsakorn
Suwanpitak, Siriwal
Pattanapanyasat, Kovit
Sermsathanasawadi, Nuttawut
Wattanapanitch, Methichit
CELL AND TISSUE RESEARCH, 2022, 388 (01) : 89 - 104
[35] Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
Choi, In Young
Lim, HoTae
Lee, Gabsang
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2014, (86):
[36] Generation of β cells from human pluripotent stem cells: Are we there yet?
Schiesser, Jacqueline V.
Wells, James M.
YEAR IN DIABETES AND OBESITY, 2014, 1311 : 124 - 137
[37] Generation of mature hematopoietic cells from human pluripotent stem cells
Padma Priya Togarrati
Kran Suknuntha
International Journal of Hematology, 2012, 95 : 617 - 623
[38] Generation of mature hematopoietic cells from human pluripotent stem cells
Togarrati, Padma Priya
Suknuntha, Kran
INTERNATIONAL JOURNAL OF HEMATOLOGY, 2012, 95 (06) : 617 - 623
[39] Highly Efficient Generation of Human Hepatocyte-Like Cells from Induced Pluripotent Stem Cells
Si-Tayeb, Karim
Noto, Fallon K.
Nagaoka, Masato
Li, Jixuan
Battle, Michele A.
Duris, Christine
North, Paula E.
Dalton, Stephen
Duncan, Stephen A.
HEPATOLOGY, 2010, 51 (01) : 297 - 305
[40] High-Efficient Generation of Induced Pluripotent Stem Cells from Human Astrocytes
Ruiz, Sergio
Brennand, Kristen
Panopoulos, Athanasia D.
Herrerias, Aida
Gage, Fred H.
Carlos Izpisua-Belmonte, Juan
PLOS ONE, 2010, 5 (12):

← 1 2 3 4 5 →