Protocol for efficient generation of human artery and vein endothelial cells from pluripotent stem cells

被引：1

作者：

Loh, Kyle M. ^{[1
,2
]}

Zheng, Sherry Li ^{[1
,2
]}

Liu, Kevin J. ^{[1
]}

Yin, Qingqing ^{[1
,2
]}

Amir-Ugokwe, Zhainib A. ^{[1
,3
]}

Jha, Sawan K. ^{[3
]}

Qi, Yue ^{[4
]}

Wazny, Vanessa K. ^{[5
]}

Nguyen, Alana T. ^{[1
,8
]}

Chen, Angela ^{[1
,9
]}

Njunkeng, Faith-Masong ^{[6
]}

Cheung, Christine ^{[5
,7
]}

Spiekerkoetter, Edda

Red-Horse, Kristy ^{[1
,3
]}

Ang, Lay Teng ^{[1
,6
]}

机构：

[1] Stanford Univ, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Dev Biol, Stanford, CA 94305 USA

[3] Stanford Univ, Howard Hughes Med Inst, Dept Biol, Stanford, CA 94305 USA

[4] Stanford Univ, Vera Moulton Wall Ctr Pulm Vasc Dis, Dept Med, Div Pulm Allergy & Crit Care Med, Stanford, CA 94305 USA

[5] Nanyang Technol Univ, Lee Kong Chian Sch Med, Singapore 308232, Singapore

[6] Stanford Univ, Dept Urol, Stanford, CA 94305 USA

[7] ASTAR, Inst Mol & Cell Biol, Singapore 138673, Singapore

[8] Univ Calif Davis, Sch Vet Med, Davis, CA 95616 USA

[9] Orca Bio, Menlo Pk, CA 94025 USA

来源：

STAR PROTOCOLS | 2025年 / 6卷 / 01期

关键词：

BONE MORPHOGENETIC PROTEIN-4; MESODERM FORMATION; PRIMITIVE STREAK; DIFFERENTIATION; INHIBITORS; SPECIFICATION; ACTIVATION; KINASE; VEGF; DISSOCIATION;

D O I：

10.1016/j.xpro.2024.103494

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Blood vessels permeate all organs and execute myriad roles in health and disease. Here, we present a protocol to efficiently generate human artery and vein endothelial cells (ECs) from pluripotent stem cells within 3-4 days of differentiation. We delineate how to seed human pluripotent stem cells and sequentially differentiate them into primitive streak, lateral mesoderm, and either artery or vein ECs. We differentiate stem cells in defined, serum-free culture media in monolayers, without feeder cells or genetic manipulations. For complete details on the use and execution of this protocol, please refer to Ang et al.

引用

页数：38

共 50 条

[1] Rapid and Highly Efficient Generation of Induced Pluripotent Stem Cells from Human Umbilical Vein Endothelial Cells
Panopoulos, Athanasia D.
Ruiz, Sergio
Yi, Fei
Herrerias, Aida
Batchelder, Erika M.
Carlos, Juan
Belmonte, Izpisua
PLOS ONE, 2011, 6 (05):
[2] Efficient generation of endothelial cells from human pluripotent stem cells and characterization of their functional properties
Song, Wei
Kaufman, Dan S.
Shen, Wei
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2016, 104 (03) : 678 - 687
[3] High efficient generation of neural stem cells from human pluripotent stem cells
Yan, Y.
Shin, S.
Sams, A.
Vemuri, M. C.
JOURNAL OF NEUROCHEMISTRY, 2013, 125 : 175 - 176
[4] Building human artery and vein endothelial cells from pluripotent stem cells, and enduring mysteries surrounding arteriovenous development
Loh, Kyle M.
Ang, Lay Teng
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2024, 155 : 62 - 75
[5] Generation of vascular endothelial and smooth muscle cells from human pluripotent stem cells
Christoph Patsch
Ludivine Challet-Meylan
Eva C. Thoma
Eduard Urich
Tobias Heckel
John F. O’Sullivan
Stephanie J. Grainger
Friedrich G. Kapp
Lin Sun
Klaus Christensen
Yulei Xia
Mary H. C. Florido
Wei He
Wei Pan
Michael Prummer
Curtis R. Warren
Roland Jakob-Roetne
Ulrich Certa
Ravi Jagasia
Per-Ola Freskgård
Isaac Adatto
Dorothee Kling
Paul Huang
Leonard I. Zon
Elliot L. Chaikof
Robert E. Gerszten
Martin Graf
Roberto Iacone
Chad A. Cowan
Nature Cell Biology, 2015, 17 : 994 - 1003
[6] Generation of Endothelial Cells From Human Pluripotent Stem Cells Methods, Considerations, and Applications
Williams, Ian M.
Wu, Joseph C.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2019, 39 (07) : 1317 - 1329
[7] Generation of vascular endothelial and smooth muscle cells from human pluripotent stem cells
Patsch, Christoph
Challet-Meylan, Ludivine
Thoma, Eva C.
Urich, Eduard
Heckel, Tobias
O'Sullivan, John F.
Grainger, Stephanie J.
Kapp, Friedrich G.
Sun, Lin
Christensen, Klaus
Xia, Yulei
Florido, Mary H. C.
He, Wei
Pan, Wei
Prummer, Michael
Warren, Curtis R.
Jakob-Roetne, Roland
Certa, Ulrich
Jagasia, Ravi
Freskgard, Per-Ola
Adatto, Isaac
Kling, Dorothee
Huang, Paul
Zon, Leonard I.
Chaikof, Elliot L.
Gerszten, Robert E.
Graf, Martin
Iacone, Roberto
Cowan, Chad A.
NATURE CELL BIOLOGY, 2015, 17 (08) : 994 - U294
[8] Generation of induced pluripotent stem cells from human umbilical cord vein mesenchymal stem cells
Fathi, Fardin
Abdi, Mehdad
Zare, Sonia
Anjamrooz, Seyed Hadi
Rezaei, Mohammad Jafar
Rostamzadeh, Jalal
TRANSGENIC RESEARCH, 2016, 25 (02) : 231 - 231
[9] Efficient generation of functional pancreatic β-cells from human induced pluripotent stem cells
Yabe, Shigeharu G.
Fukuda, Satsuki
Takeda, Fujie
Nashiro, Kiyoko
Shimoda, Masayuki
Okochi, Hitoshi
JOURNAL OF DIABETES, 2017, 9 (02) : 168 - 179
[10] Efficient generation of lung and airway epithelial cells from human pluripotent stem cells
Sarah X L Huang
Mohammad Naimul Islam
John O'Neill
Zheng Hu
Yong-Guang Yang
Ya-Wen Chen
Melanie Mumau
Michael D Green
Gordana Vunjak-Novakovic
Jahar Bhattacharya
Hans-Willem Snoeck
Nature Biotechnology, 2014, 32 : 84 - 91

← 1 2 3 4 5 →