Effects of DCA gene overexpression in transgenic Dunaliella parva

被引：0
|
作者：
Ruan, Lingru [1 ,2 ,3 ]
Zhou, Bingfeng [1 ,2 ,3 ]
Liang, Yanyan [1 ,2 ,3 ]
Zhang, Xiumin [1 ,2 ,3 ]
Wu, Lina [1 ,2 ,3 ]
Huang, Limei [1 ,2 ,3 ]
Liang, Shiyu [1 ,2 ,3 ]
Shang, Changhua [1 ,2 ,3 ]
机构：
[1] Guangxi Normal Univ, Key Lab Ecol Rare & Endangered Species & Environm, Minist Educ, 1,Yanzhong Rd, Guilin 541006, Guangxi, Peoples R China
[2] Guangxi Normal Univ, Guangxi Key Lab Landscape Resources Conservat & Su, 1,Yanzhong Rd, Guilin 541006, Guangxi, Peoples R China
[3] Univ Engn Res Ctr Bioinformat & Genet Improvement, 1,Yanzhong Rd, Guilin 541006, Guangxi, Peoples R China
来源：
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 2025年 / 118卷 / 02期
关键词：
<italic>Dunaliella parva</italic>; <italic>DpDCA</italic> gene; Organic content; Enzymatic activity; Gene expression; EXTRACELLULAR CARBONIC-ANHYDRASE; CONCENTRATING MECHANISMS; MODULATION; LOCATIONS; EVOLUTION; DISCOVERY; STRESS; GROWTH; PLANTS; BETA;
D O I：
10.1007/s10482-024-02054-9
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Duplicated carbonic anhydrase was a member of carbonic anhydrase family. The ubiquitous carbonic anhydrase (CA) plays important roles in plants. However, the role of CA in Dunaliella parva remains to be further explored. In this study, a member of the CAs family was isolated from D. parva and named DpDCA. It was transferred into D. parva by genetic engineering techniques. The carotenoid, total carbohydrate, starch, protein, and oil contents of transgenic D. parva increased by 16.31%, 31.68%, 43.97%, 52.91%, and 12.32%, compared to control. Perhaps DpDCA affected photosynthesis through cis-element. Therefore, this study lays a foundation for studying the function of DpDCA gene in D. parva.
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页数：9
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