Effects of DCA gene overexpression in transgenic Dunaliella parva

被引：0

作者：

Ruan, Lingru ^{[1
,2
,3
]}

Zhou, Bingfeng ^{[1
,2
,3
]}

Liang, Yanyan ^{[1
,2
,3
]}

Zhang, Xiumin ^{[1
,2
,3
]}

Wu, Lina ^{[1
,2
,3
]}

Huang, Limei ^{[1
,2
,3
]}

Liang, Shiyu ^{[1
,2
,3
]}

Shang, Changhua ^{[1
,2
,3
]}

机构：

[1] Guangxi Normal Univ, Key Lab Ecol Rare & Endangered Species & Environm, Minist Educ, 1,Yanzhong Rd, Guilin 541006, Guangxi, Peoples R China

[2] Guangxi Normal Univ, Guangxi Key Lab Landscape Resources Conservat & Su, 1,Yanzhong Rd, Guilin 541006, Guangxi, Peoples R China

[3] Univ Engn Res Ctr Bioinformat & Genet Improvement, 1,Yanzhong Rd, Guilin 541006, Guangxi, Peoples R China

来源：

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 2025年 / 118卷 / 02期

关键词：

<italic>Dunaliella parva</italic>; <italic>DpDCA</italic> gene; Organic content; Enzymatic activity; Gene expression; EXTRACELLULAR CARBONIC-ANHYDRASE; CONCENTRATING MECHANISMS; MODULATION; LOCATIONS; EVOLUTION; DISCOVERY; STRESS; GROWTH; PLANTS; BETA;

D O I：

10.1007/s10482-024-02054-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Duplicated carbonic anhydrase was a member of carbonic anhydrase family. The ubiquitous carbonic anhydrase (CA) plays important roles in plants. However, the role of CA in Dunaliella parva remains to be further explored. In this study, a member of the CAs family was isolated from D. parva and named DpDCA. It was transferred into D. parva by genetic engineering techniques. The carotenoid, total carbohydrate, starch, protein, and oil contents of transgenic D. parva increased by 16.31%, 31.68%, 43.97%, 52.91%, and 12.32%, compared to control. Perhaps DpDCA affected photosynthesis through cis-element. Therefore, this study lays a foundation for studying the function of DpDCA gene in D. parva.

引用

页数：9

共 50 条

[31] THE PHOTOSYNTHESIS OF DUNALIELLA-PARVA LERCHE AS A FUNCTION OF TEMPERATURE, LIGHT AND SALINITY
JIMENEZ, C
NIELL, FX
FERNANDEZ, JA
HYDROBIOLOGIA, 1990, 197 : 165 - 172
[32] Discovery of genes for production of biofuels through transcriptome sequencing of Dunaliella parva
Shang, Changhua
Bi, Guican
Yuan, Zhenhong
Wang, Zhongming
Alam, Mohammad Asraful
Xie, Jun
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 2016, 13 : 318 - 326
[33] LOW PERMEABILITY OF THE PLASMA-MEMBRANE OF DUNALIELLA-PARVA FOR SOLUTES
GIMMLER, H
HARTUNG, W
JOURNAL OF PLANT PHYSIOLOGY, 1988, 133 (02) : 165 - 172
[34] Transformation of Dunaliella parva with PSY gene: Carotenoids show enhanced antioxidant activity under polyethylene glycol and calcium treatments
Ismaiel, Mostafa M. S.
El-Ayouty, Yassin M.
Said, Alaa. A.
Fathey, Hoda A.
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 2018, 16 : 378 - 384
[35] POPULATION-DYNAMICS OF DUNALIELLA-PARVA IN THE DEAD-SEA
OREN, A
SHILO, M
LIMNOLOGY AND OCEANOGRAPHY, 1982, 27 (02) : 201 - 211
[36] Asymmetric reduction of hydroxyacetone to propanediol in immobilized halotolerant microalga Dunaliella parva
Hatanaka, Y
Kudo, T
Miyataka, M
Kobayashi, O
Higashihara, M
Hiyama, K
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 1999, 88 (03) : 281 - 286
[37] LIGHT-INDUCED PROTON UPTAKE IN WHOLE CELLS OF DUNALIELLA PARVA
BENAMOTZ, A
GINZBURG, BZ
BIOCHIMICA ET BIOPHYSICA ACTA, 1969, 183 (01) : 144 - &
[38] ROLE OF GLYCEROL IN OSMOTIC REGULATION OF HALOPHILIC ALGA DUNALIELLA-PARVA
BENAMOTZ, A
AVRON, M
PLANT PHYSIOLOGY, 1973, 51 (05) : 875 - 878
[39] MEASUREMENTS OF ION CONCENTRATIONS IN DUNALIELLA-PARVA SUBJECTED TO HYPERTONIC SHOCK
GINZBURG, M
JOURNAL OF EXPERIMENTAL BOTANY, 1981, 32 (127) : 333 - 340
[40] A duplicated carbonic anhydrase 1 (DCA1) promoter mediates the nitrate reductase gene switch of Dunaliella salina
Yumin Lu
Jie Li
Lexun Xue
Hongxia Yan
Huijuan Yuan
Cui Wang
Journal of Applied Phycology, 2011, 23 : 673 - 680

← 1 2 3 4 5 →