Real time detection of mRNA transcripts in vivo using two-photon resonance energy transfer imaging

被引:0
|
作者
Tsourkas, Andrew [1 ]
Berland, Keith [1 ]
Bao, Gang [1 ]
机构
[1] Biomedical Engineering Soc, Atlanta, United States
关键词
Fluorescence - Molecular biology - Molecules - Photons - RNA;
D O I
暂无
中图分类号
学科分类号
摘要
A novel approach for the real detection of single mRNA molecules in vivo by combining new FRET-based probes with two-photon imaging is presented. This approach allows the fluorescence from multiple probes on a single mRNA to be observed as a point source.
引用
收藏
相关论文
共 50 条
  • [41] Two-Photon In Vivo Imaging with Porous Silicon Nanoparticles
    Kim, Dokyoung
    Kang, Jinyoung
    Wang, Taejun
    Ryu, Hye Gun
    Zuidema, Jonathan M.
    Joo, Jinmyoung
    Kim, Muwoong
    Huh, Youngbuhm
    Jung, Junyang
    Ahn, Kyo Han
    Kim, Ki Hean
    Sailor, Michael J.
    ADVANCED MATERIALS, 2017, 29 (39)
  • [42] Real-time cellular uptake of serotonin using fluorescence lifetime imaging with two-photon excitation
    Botchway, Stanley Walter
    Parker, Anthony William
    Bisby, Roger Hugh
    Crisostomo, Ana Goncalves
    MICROSCOPY RESEARCH AND TECHNIQUE, 2008, 71 (04) : 267 - 273
  • [43] A New Model For Real Time Intravital Imaging Of Airway Inflammation In The Mouse Using Two-Photon Microscopy
    Pieper, M.
    Kretschmer, S.
    Martensen, B.
    Huettmann, G.
    Koenig, P.
    AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2011, 183
  • [44] In vivo imaging of the human retina using a two-photon excited fluorescence ophthalmoscope
    Boguslawski, Jakub
    Tomczewski, Slawomir
    Dabrowski, Michal
    Komar, Katarzyna
    Milkiewicz, Jadwiga
    Palczewska, Grazyna
    Palczewski, Krzysztof
    Wojtkowski, Maciej
    STAR PROTOCOLS, 2023, 4 (02):
  • [45] Monitoring the release of a NO photodonor from polymer nanoparticles via Forster resonance energy transfer and two-photon fluorescence imaging
    Conte, Claudia
    Fraix, Aurore
    Thomsen, Hanna
    Ungaro, Francesca
    Cardile, Venera
    Graziano, Adriana C. E.
    Ericson, Marica B.
    Quaglia, Fabiana
    Sortino, Salvatore
    JOURNAL OF MATERIALS CHEMISTRY B, 2018, 6 (02) : 249 - 256
  • [46] Spectra and lifetimes of fluorescence resonance energy transfer fluorophores under two-photon excitation
    Ashman, R
    Crimeen-Irwin, B
    Ludford-Menting, M
    Russell, S
    Gu, M
    SCANNING, 2003, 25 (03) : 116 - 120
  • [47] Characterization of one- and two-photon excitation fluorescence resonance energy transfer microscopy
    Elangovan, M
    Wallrabe, H
    Chen, Y
    Day, RN
    Barroso, M
    Periasamy, A
    METHODS, 2003, 29 (01) : 58 - 73
  • [48] In vivo imaging of cerebral energy metabolism with two-photon fluorescence lifetime microscopy of NADH
    Yaseen, Mohammad A.
    Sakadzic, Sava
    Wu, Weicheng
    Becker, Wolfgang
    Kasischke, Karl A.
    Boas, David A.
    BIOMEDICAL OPTICS EXPRESS, 2013, 4 (02): : 307 - 321
  • [49] A genetically encoded Forster resonance energy transfer biosensor for two-photon excitation microscopy
    Kumagai, Yuka
    Kamioka, Yuji
    Yagi, Shunsuke
    Matsuda, Michiyuki
    Kiyokawa, Etsuko
    ANALYTICAL BIOCHEMISTRY, 2011, 413 (02) : 192 - 199
  • [50] NIR two-photon fluorescent probe for biothiol detection and imaging of living cells in vivo
    Xia, Xiang
    Qian, Ying
    ANALYST, 2018, 143 (21) : 5218 - 5224