Real time detection of mRNA transcripts in vivo using two-photon resonance energy transfer imaging

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作者
Tsourkas, Andrew [1 ]
Berland, Keith [1 ]
Bao, Gang [1 ]
机构
[1] Biomedical Engineering Soc, Atlanta, United States
关键词
Fluorescence - Molecular biology - Molecules - Photons - RNA;
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摘要
A novel approach for the real detection of single mRNA molecules in vivo by combining new FRET-based probes with two-photon imaging is presented. This approach allows the fluorescence from multiple probes on a single mRNA to be observed as a point source.
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