Labelling of proteins by use of iodination and detection by ICP-MS

被引：0

作者：

Jakubowski, Norbert ^{[1
]}

Messerschmidt, Juergen ^{[1
]}

Añorbe, Marta Garijo ^{[1
]}

Waentig, Larissa ^{[1
]}

Hayen, Heiko ^{[1
]}

Roos, Peter H. ^{[2
]}

机构：

[1] Institute for Analytical Sciences (ISAS), Bunsen-Kirchhoff-Str. 11, D-44139, Dortmund, Germany

[2] Leibniz Research Centre for Working Environment and Human Factors, Technical University of Dortmund (IfADo), Ardeystr. 67, D-44139, Dortmund, Germany

来源：

Journal of Analytical Atomic Spectrometry | 2008年 / 23卷 / 11期

关键词：

The labelling of three different proteins; bovine serum albumin (BSA); chicken egg white lysozyme and porcine gastric mucosa pepsin; with iodine using a commercially available reaction kit has been investigated for total protein amounts of 500 μg. The assay described here has already often been applied but with radioactive iodine isotopes and is extended in this work for the use of the stable isotope 127I and detection by ICP-MS. The reaction conditions are investigated and optimised for proteins separated by SDS-PAGE and electroblotted onto NC membranes. Detection has been performed by laser ablation ICP-MS on the membranes. Long reaction times result in degradation of proteins; whereas for short reaction times (4 min) integrated sensitivities of even 108 cps pmol-1 of protein have been realized. Total amounts of proteins have been investigated in the range from 0.015 (BSA) to 105 pmol (lysozyme). A calibration was performed for BSA from 0.015 to 15 pmol but the limit of detection was reached already at about 150 fmol due to high iodine blank values; only. The labelling procedure described here does not affect protein mobility in SDS-PAGE; because the change of the molecular weight is very moderate for the proteins investigated here. This is quite opposite to those procedures where chelating compounds are applied. ESI-FTICR-MS has been used to identify histidine and tyrosine as the target amino acids for iodination. Additionally; oxidation of methionine residues has been observed. © The Royal Society of Chemistry 2008;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Journal article (JA)

引用

页码：1487 / 1496

共 50 条

[41] Strategies for Achieving the Lowest Possible Detection Limits in ICP-MS
Chemnitzer, Rene
SPECTROSCOPY, 2019, 34 (10) : 12 - 16
[42] Method development for metal detection at cellular levels by ICP-MS
Jakuboski, Norbert
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2018, 255
[43] Optimization of photochemical vapor generation of nickel with ICP-MS detection
Soukal, Jakub
Musil, Stanislav
PROCEEDINGS OF THE 15TH INTERNATIONAL STUDENTS CONFERENCE MODERN ANALYTICAL CHEMISTRY, 2019, : 179 - 183
[44] Innovations in speciation analysis using HPLC with ICP-MS detection
Neubauer, Kenneth
SPECTROSCOPY, 2008, 23 (04) : 22 - +
[45] Application of ICP-MS to the detection of heavy metals in transgenic corn
Rui Yu-kui
Guo Jing
Huang Kun-Lun
Jin Yin-hua
Luo Yun-bo
SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27 (04) : 796 - 798
[46] A novel detection scheme of trace elements using ICP-MS
Yau, MHP
Chan, WT
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 2005, 20 (11) : 1197 - 1202
[47] Method detection limits for trace metals analysis by ICP-MS
Ketkar, SN
Bzik, TJ
INSTITUTE OF ENVIRONMENTAL SCIENCES AND TECHNOLOGY, 1998 PROCEEDINGS - CONTAMINATION CONTROL, 1998, : 377 - 384
[48] When Size Matters: ICP-MS Detection of Small Objects
Corte-Rodriguez, Mario
Montes-Bayon, Maria
Bettmer, Jorg
SPECTROSCOPY, 2020, 35 (07) : 23 - 24
[49] Trends in single-cell analysis by use of ICP-MS
Mueller, Larissa
Traub, Heike
Jakubowski, Norbert
Drescher, Daniela
Baranov, Vladimir I.
Kneipp, Janina
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2014, 406 (27) : 6963 - 6977
[50] Nano-particle labelling of nucleic acids for enhanced detection by inductively-coupled plasma mass spectrometry (ICP-MS)
Kerr, Samantha Louise
Sharp, Barry
CHEMICAL COMMUNICATIONS, 2007, (43) : 4537 - 4539

← 1 2 3 4 5 →