Labelling of proteins by use of iodination and detection by ICP-MS

被引：0

作者：

Jakubowski, Norbert ^{[1
]}

Messerschmidt, Juergen ^{[1
]}

Añorbe, Marta Garijo ^{[1
]}

Waentig, Larissa ^{[1
]}

Hayen, Heiko ^{[1
]}

Roos, Peter H. ^{[2
]}

机构：

[1] Institute for Analytical Sciences (ISAS), Bunsen-Kirchhoff-Str. 11, D-44139, Dortmund, Germany

[2] Leibniz Research Centre for Working Environment and Human Factors, Technical University of Dortmund (IfADo), Ardeystr. 67, D-44139, Dortmund, Germany

来源：

Journal of Analytical Atomic Spectrometry | 2008年 / 23卷 / 11期

关键词：

The labelling of three different proteins; bovine serum albumin (BSA); chicken egg white lysozyme and porcine gastric mucosa pepsin; with iodine using a commercially available reaction kit has been investigated for total protein amounts of 500 μg. The assay described here has already often been applied but with radioactive iodine isotopes and is extended in this work for the use of the stable isotope 127I and detection by ICP-MS. The reaction conditions are investigated and optimised for proteins separated by SDS-PAGE and electroblotted onto NC membranes. Detection has been performed by laser ablation ICP-MS on the membranes. Long reaction times result in degradation of proteins; whereas for short reaction times (4 min) integrated sensitivities of even 108 cps pmol-1 of protein have been realized. Total amounts of proteins have been investigated in the range from 0.015 (BSA) to 105 pmol (lysozyme). A calibration was performed for BSA from 0.015 to 15 pmol but the limit of detection was reached already at about 150 fmol due to high iodine blank values; only. The labelling procedure described here does not affect protein mobility in SDS-PAGE; because the change of the molecular weight is very moderate for the proteins investigated here. This is quite opposite to those procedures where chelating compounds are applied. ESI-FTICR-MS has been used to identify histidine and tyrosine as the target amino acids for iodination. Additionally; oxidation of methionine residues has been observed. © The Royal Society of Chemistry 2008;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Journal article (JA)

引用

页码：1487 / 1496

共 50 条

[31] Application of ICP-MS to the detection of forty elements in wine
Rui Yu-kui
Yu Qing-quan
Jin Yin-hua
Guo Jing
Luo Yun-bo
SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27 (05) : 1015 - 1017
[32] ICP-MS Detection for HPLC Analyses of Pharmaceutical Products
Shelbourn, Tim
Williamson, Leah
Gillespie, Todd
Montgomery, Robert
LC GC NORTH AMERICA, 2009, 27 (02) : 164 - +
[33] Simultaneous determination of proteins using an element-tagged immunoassay coupled with ICP-MS detection
Quinn, ZA
Baranov, VI
Tanner, SD
Wrana, JL
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 2002, 17 (08) : 892 - 896
[34] “Heteroatom-tagged” quantification of proteins via ICP-MS
Alfredo Sanz-Medel
Analytical and Bioanalytical Chemistry, 2016, 408 : 5393 - 5395
[35] Single-cell analysis by use of ICP-MS
Theiner, Sarah
Loehr, Konrad
Koellensperger, Gunda
Mueller, Larissa
Jakubowski, Norbert
Journal of Analytical Atomic Spectrometry, 2020, 35 (09): : 1784 - 1813
[36] Single-cell analysis by use of ICP-MS
Theiner, Sarah
Loehr, Konrad
Koellensperger, Gunda
Mueller, Larissa
Jakubowski, Norbert
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY, 2020, 35 (09) : 1784 - 1813
[37] Guide for determining ICP/ICP-MS method detection limits and instrument performance
Sivakumar, Vanaja
Ernyei, Laszlo
Obenauf, Ralph H.
SPECTROSCOPY, 2006, : 13 - 13
[38] Nanoparticle measurements using single particle ICP-MS and capillary electrophoresis ICP-MS
Olesik, John
Jiao, Shi
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2017, 254
[39] Quantification of proteins in whole blood, plasma and DBS, with element-labelled antibody detection by ICP-MS
Hogeling, Shanna M.
Cox, Michael T.
Bradshaw, Robert M.
Smith, David P.
Duckett, Catherine J.
ANALYTICAL BIOCHEMISTRY, 2019, 575 : 10 - 16
[40] 99Tc detection in water samples by ICP-MS
Mas, JL
García-León, M
Bolívar, JP
RADIOCHIMICA ACTA, 2004, 92 (01) : 39 - 46

← 1 2 3 4 5 →