Isolation and oligomeric composition of cytochrome c nitrite reductase from the haloalkaliphilic bacterium Thioalkalivibrio nitratireducens

被引：0
|
作者：
Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky pr. 33, Moscow 119071, Russia [1 ]
不详 [2 ]
不详 [3 ]
不详 [4 ]
机构：
来源：
Biochemistry Moscow | 2008年 / 2卷 / 164-170期
基金：
俄罗斯基础研究基金会;
关键词：
Bacteria - Gel permeation chromatography - Oligomers - Purification - Thermodynamic stability - X ray scattering;
D O I：
10.1007/s10541-008-2007-8
中图分类号：
学科分类号：
摘要：
A new procedure for isolation of cytochrome c nitrite reductase from the haloalkaliphilic bacterium Thioalkalivibrio nitratireducens increasing significantly the yield of the purified enzyme is presented. The enzyme is isolated from the soluble fraction of the cell extract as a hexamer, as shown by gel filtration chromatography and small angle X-ray scattering analysis. Thermostability of the hexameric form of the nitrite reductase is characterized in terms of thermoinactivation and thermodenaturation. © 2008 MAIK Nauka.
引用
收藏
相关论文
共 50 条
  • [21] Nitrite Reductase Activity of Cytochrome c
    Basu, Swati
    Azarova, Natalia A.
    Font, Michael D.
    King, S. Bruce
    Hogg, Neil
    Gladwin, Mark T.
    Shiva, Sruti
    Kim-Shapiro, Daniel B.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (47) : 32590 - 32597
  • [22] Structure of cytochrome c nitrite reductase
    Nature, 6743 (476-480):
  • [23] Nitrite Reductase Activity of Cytochrome C
    Basu, Swati
    Azarova, Natalia A.
    Font, Michael D.
    King, S. Bruce
    Hogg, Neil
    Gladwin, Mark T.
    Shiva, Sruti
    Kim-Shapiro, Daniel B.
    FREE RADICAL BIOLOGY AND MEDICINE, 2008, 45 : S110 - S110
  • [24] Structure of cytochrome c nitrite reductase
    Oliver Einsle
    Albrecht Messerschmidt
    Petra Stach
    Gleb P. Bourenkov
    Hans D. Bartunik
    Robert Huber
    Peter M. H. Kroneck
    Nature, 1999, 400 : 476 - 480
  • [25] Cytochrome c nitrite reductase:: from structural to physicochemical analysis
    Burlat, B
    Gwyer, JD
    Poock, S
    Clarke, T
    Cole, JA
    Hemmings, AM
    Cheesman, MR
    Butt, JN
    Richardson, DJ
    BIOCHEMICAL SOCIETY TRANSACTIONS, 2005, 33 : 137 - 140
  • [26] CYTOCHROME-C-OXIDASE ACTIVITY OF THE COPPER-CONTAINING NITRITE REDUCTASE OF A PHOTOSYNTHETIC BACTERIUM
    ISHIGURO, K
    SAKAI, T
    GOTO, M
    JOURNAL OF PHARMACOBIO-DYNAMICS, 1987, 10 (01): : S17 - S17
  • [27] Structural adaptations of octaheme nitrite reductases from haloalkaliphilic Thioalkalivibrio bacteria to alkaline pH and high salinity
    Popinako, Anna
    Antonov, Mikhail
    Tikhonov, Alexey
    Tikhonova, Tamara
    Popov, Vladimir
    PLOS ONE, 2017, 12 (05):
  • [28] Nitrite reductase activity of hemoglobin and cytochrome c
    Kim-Shapiro, Daniel
    NITRIC OXIDE-BIOLOGY AND CHEMISTRY, 2009, 20 : S23 - S23
  • [29] The nitric oxide reductase activity of cytochrome c nitrite reductase from Escherichia coli
    van Wonderen, Jessica H.
    Burlat, Benedicte
    Richardson, David J.
    Cheesman, Myles R.
    Butt, Julea N.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (15) : 9587 - 9594
  • [30] Crystal structure of cytochrome c nitrite reductase from Sulfurospirillum deleyianum
    Einsle, O
    Messerschmidt, A
    Stach, P
    Huber, R
    Kroneck, PMH
    JOURNAL OF INORGANIC BIOCHEMISTRY, 1999, 74 (1-4) : 121 - 121
12345