Isolation and oligomeric composition of cytochrome c nitrite reductase from the haloalkaliphilic bacterium Thioalkalivibrio nitratireducens

被引：0
|
作者：
Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky pr. 33, Moscow 119071, Russia [1 ]
不详 [2 ]
不详 [3 ]
不详 [4 ]
机构：
来源：
Biochemistry Moscow | 2008年 / 2卷 / 164-170期
基金：
俄罗斯基础研究基金会;
关键词：
Bacteria - Gel permeation chromatography - Oligomers - Purification - Thermodynamic stability - X ray scattering;
D O I：
10.1007/s10541-008-2007-8
中图分类号：
学科分类号：
摘要：
A new procedure for isolation of cytochrome c nitrite reductase from the haloalkaliphilic bacterium Thioalkalivibrio nitratireducens increasing significantly the yield of the purified enzyme is presented. The enzyme is isolated from the soluble fraction of the cell extract as a hexamer, as shown by gel filtration chromatography and small angle X-ray scattering analysis. Thermostability of the hexameric form of the nitrite reductase is characterized in terms of thermoinactivation and thermodenaturation. © 2008 MAIK Nauka.
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