Progress in the Research of Synthetic Biology in Construction of Engineered Microorganisms

被引：1
|
作者：
Gao X. [1 ]
Liu Y. [1 ]
Jiang Z. [1 ]
Zeng Q. [1 ]
Liu S. [1 ]
Liu X. [1 ]
Min W. [1 ]
机构：
[1] National Engineering Laboratory on Wheat and Corn Further Processing, College of Food Science and Engineering, Jilin Agricultural University, Changchun
来源：
Shipin Kexue/Food Science | 2022年 / 43卷 / 15期
关键词：
Construction of engineered microorganisms; Genome editing; Metabolic regulation; Synthetic biology;
D O I：
10.7506/spkx1002-6630-20210831-414
中图分类号：
学科分类号：
摘要：
As a new interdisciplinary subject that covers many fields in the 21st century, synthetic biology is of great help in exploring the basic laws of life activities and biotechnological innovations and breakthroughs. Synthetic biotechnology is a new approach to construct engineered microorganisms. It has been widely used to produce amino acids, organic acids, aromatic compounds, sugars, and so on. The yield of target products produced by recombinant engineered microorganisms can be efficiently increased by using advanced modules, systematic design and new genome editing methods to engineer the complex metabolic pathways in type strains, promoting the rapid development of social productivity. In this paper, the latest advances in the application of synthetic biology for the construction of engineered microorganism are reviewed, the developmental course of synthetic biotechnology is elaborated, and its application for the construction of engineered microorganism is exemplified. With the deepening of research on engineered microorganisms, synthetic biotechnology will bring new breakthroughs and opportunities for the fermentation industry. © 2022, China Food Publishing Company. All right reserved.
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页码：256 / 264
页数：8
相关论文
共 54 条
  • [1] GARDNER T S, CANTOR C R, COLLINS J J., Construction of a genetic toggle switch in Escherichia coli, Nature, 403, pp. 339-342, (2000)
  • [2] LE Cong, LIN Shuailiang, DAVID C, Et al., Multiplex genome engineering using CRISPR/Cas systems, Science, 339, pp. 819-823, (2013)
  • [3] BERND Z, JONATHAN S G, OMAR O A, Et al., Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system, Cell, 163, 3, pp. 759-771, (2015)
  • [4] KRUMBACH K, SONNTAG C K, EGGELING L, Et al., CRISPR/Cas12a mediated genome editing to introduce amino acid substitutions into the mechanosensitive channel MscCG of Corynebacterium glutamicum, ACS Synthetic Biology, 8, 12, pp. 2726-2734, (2019)
  • [5] DING Dan, CHEN Kaiyuan, CHEN Yuedan, Et al., Engineering introns to express RNA guides for Cas9- and Cpf1-mediated multiplex genome editing, Molecular Plant, 11, 4, pp. 542-552, (2018)
  • [6] FERENCZI A, PYOTT D E, XIPNITOU A, Et al., Efficient targeted DNA editing and replacement in Chlamydomonas reinhardtii using Cpf1 ribonucleoproteins and single-stranded DNA, Proceedings of the National Academy of Sciences of the United States of America, 114, 51, pp. 13567-13572, (2017)
  • [7] DANIEL G G, GWYNEDD A B, CYNTHIA A P, Et al., Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome, Science, 319, pp. 1215-1220, (2008)
  • [8] HUTCHISON C A, CHUANG R Y, NOSKOV V N, Et al., Design and synthesis of a minimal bacterial genome[J], Science, 351, (2016)
  • [9] SHEN Yue, WANG Yun, CHEN Tai, Et al., Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome, Science, 355, (2017)
  • [10] SHAO Yangyang, LU Ning, WU Zhenfang, Et al., Creating a functional single-chromosome yeast, Nature, 560, pp. 331-335, (2018)
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