Maize mutant screens: from classical methods to new CRISPR-based approaches

被引:0
|
作者
Lorenzo, Christian Damian [1 ,2 ]
Blasco-Escamez, David [1 ,2 ]
Beauchet, Arthur [1 ,2 ]
Wytynck, Pieter [1 ,2 ]
Sanches, Matilde [1 ,2 ]
del Campo, Jose Rodrigo Garcia [1 ,2 ]
Inze, Dirk [1 ,2 ]
Nelissen, Hilde [1 ,2 ]
机构
[1] VIB, Ctr Plant Syst Biol, B-9052 Ghent, Belgium
[2] Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium
基金
欧洲研究理事会;
关键词
CRISPR; forward genetics; maize; mutants; reverse genetics; screenings; transposons; REGULATORS BABY-BOOM; TARGETED MUTAGENESIS; TRANSPOSABLE ELEMENTS; QUANTITATIVE TRAITS; HAPLOID INDUCTION; GENOMIC DNA; GENE; TRANSFORMATION; DOMESTICATION; SYSTEM;
D O I
10.1111/nph.20084
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Mutations play a pivotal role in shaping the trajectory and outcomes of a species evolution and domestication. Maize (Zea mays) has been a major staple crop and model for genetic research for more than 100 yr. With the arrival of site-directed mutagenesis and genome editing (GE) driven by the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), maize mutational research is once again in the spotlight. If we combine the powerful physiological and genetic characteristics of maize with the already available and ever increasing toolbox of CRISPR-Cas, prospects for its future trait engineering are very promising. This review aimed to give an overview of the progression and learnings of maize screening studies analyzing forward genetics, natural variation and reverse genetics to focus on recent GE approaches. We will highlight how each strategy and resource has contributed to our understanding of maize natural and induced trait variability and how this information could be used to design the next generation of mutational screenings.
引用
收藏
页码:384 / 393
页数:10
相关论文
共 50 条
  • [41] CRISPR-Based Genome Editing as a New Therapeutic Tool in Retinal Diseases
    Rasoulinejad, Seyed Ahmad
    Maroufi, Faezeh
    MOLECULAR BIOTECHNOLOGY, 2021, 63 (09) : 768 - 779
  • [42] Generating new CRISPR-based mouse models for different types of albinism
    Montero, Andrea
    Guardia, Ana
    Munoz, Diego
    Cantero, Marta
    Fernandez, Julia
    Fernandez, Almudena
    Montoliu, Lluis
    TRANSGENIC RESEARCH, 2020, 29 (SUPPL 1) : 20 - 20
  • [43] Identification of potential drug targets for tuberous sclerosis complex by synthetic screens combining CRISPR-based knockouts with RNAi
    Housden, Benjamin E.
    Valvezan, Alexander J.
    Kelley, Colleen
    Sopko, Richelle
    Hu, Yanhui
    Roesel, Charles
    Lin, Shuailiang
    Buckner, Michael
    Tao, Rong
    Yilmazel, Bahar
    Mohr, Stephanie E.
    Manning, Brendan D.
    Perrimon, Norbert
    SCIENCE SIGNALING, 2015, 8 (393)
  • [44] CRISPR-Based Genome Editing as a New Therapeutic Tool in Retinal Diseases
    Seyed Ahmad Rasoulinejad
    Faezeh Maroufi
    Molecular Biotechnology, 2021, 63 : 768 - 779
  • [45] Evaluating CRISPR-Based Delivery Methods and Reagents in Blueberry (Vaccinium corymbosum L.)
    Enciso-Rodriguez, Felix E.
    Benevenuto, Juliana
    Nadakuduti, Satya Swathi
    Parikh, Aalap
    Munoz, Patricio R.
    HORTSCIENCE, 2022, 57 (09) : S167 - S167
  • [46] STANDARDIZED METHODS IN IPSC FOR CRISPR-BASED EDITING AND HOMOLOGY-DIRECTED REPAIR (HDR)
    Murugan, K.
    Thommandru, B. C.
    Glenn, S.
    Woodley, J.
    Rettig, G.
    CYTOTHERAPY, 2024, 26 (06) : S230 - S230
  • [47] CRISPR-Based Approaches for the High-Throughput Characterization of Long Non-Coding RNAs
    Hazan, Joshua
    Bester, Assaf Chanan
    NON-CODING RNA, 2021, 7 (04)
  • [48] Towards Point of Care CRISPR-Based Diagnostics: From Method to Device
    Chen, Haoxiang
    Zhou, Xi
    Wang, Miao
    Ren, Lei
    JOURNAL OF FUNCTIONAL BIOMATERIALS, 2023, 14 (02)
  • [49] Genome-wide CRISPR-based gene knockout screens reveal cellular factors and pathways essential for nasopharyngeal carcinoma
    Wang, Chong
    Jiang, Sizun
    Ke, Liangru
    Zhang, Luyao
    Li, Difei
    Liang, Jun
    Narita, Yohei
    Hou, Isabella
    Chen, Chen-hao
    Wang, Liangwei
    Zhong, Qian
    Ling, Yihong
    Lv, Xing
    Xiang, Yanqun
    Guo, Xiang
    Teng, Mingxiang
    Tsao, Sai-Wah
    Gewurz, Benjamin E.
    Zeng, Mu-Sheng
    Zhao, Bo
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2019, 294 (25) : 9734 - 9745
  • [50] Identification of tumor-suppressive and oncogenic regulatory elements in the human genome using CRISPR-based functional genomic screens
    Elkon, R.
    Korkmaz, G.
    Lopes, R.
    Li, L.
    Manber, Z.
    Agami, R.
    FEBS JOURNAL, 2017, 284 : 45 - 45