Maize mutant screens: from classical methods to new CRISPR-based approaches

被引:0
|
作者
Lorenzo, Christian Damian [1 ,2 ]
Blasco-Escamez, David [1 ,2 ]
Beauchet, Arthur [1 ,2 ]
Wytynck, Pieter [1 ,2 ]
Sanches, Matilde [1 ,2 ]
del Campo, Jose Rodrigo Garcia [1 ,2 ]
Inze, Dirk [1 ,2 ]
Nelissen, Hilde [1 ,2 ]
机构
[1] VIB, Ctr Plant Syst Biol, B-9052 Ghent, Belgium
[2] Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium
基金
欧洲研究理事会;
关键词
CRISPR; forward genetics; maize; mutants; reverse genetics; screenings; transposons; REGULATORS BABY-BOOM; TARGETED MUTAGENESIS; TRANSPOSABLE ELEMENTS; QUANTITATIVE TRAITS; HAPLOID INDUCTION; GENOMIC DNA; GENE; TRANSFORMATION; DOMESTICATION; SYSTEM;
D O I
10.1111/nph.20084
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Mutations play a pivotal role in shaping the trajectory and outcomes of a species evolution and domestication. Maize (Zea mays) has been a major staple crop and model for genetic research for more than 100 yr. With the arrival of site-directed mutagenesis and genome editing (GE) driven by the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), maize mutational research is once again in the spotlight. If we combine the powerful physiological and genetic characteristics of maize with the already available and ever increasing toolbox of CRISPR-Cas, prospects for its future trait engineering are very promising. This review aimed to give an overview of the progression and learnings of maize screening studies analyzing forward genetics, natural variation and reverse genetics to focus on recent GE approaches. We will highlight how each strategy and resource has contributed to our understanding of maize natural and induced trait variability and how this information could be used to design the next generation of mutational screenings.
引用
收藏
页码:384 / 393
页数:10
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