Validation of the Sysmex XN-V hematology analyzer for feline specimens

Background: The Sysmex XN- V is derived from the new Sysmex XN series of human hematology analyzers. The main changes from the previously validated XT- 2000iV analyzer include an optic- fluorescent analysis for platelets and a nucleated red blood cell (NRBC) count. Objective: We aimed to validate the Sysmex XN- V for feline blood following the American College for Veterinary Clinical Pathology and International Council for Standardization in Hematology recommendations. Methods: Feline EDTA blood specimens were analyzed on the Sysmex XN- V to evaluate repeatability, linearity, comparison with the XT- 2000iV analyzer and manual methods, stability, and to verify the previously established Sysmex XT- 2000iV RIs. Results: Repeatability was excellent for most variables. Visually determined linearity was excellent or good for most variables except eosinophils and platelet variables. The correlation between the XN- V and XT- 2000iV analyzers was good (>= 0.82) for all variables except reticulocyte indices. Correlations between the Sysmex XN- V and manual differential counts were good to excellent for most variables, acceptable for neutrophils, and fair for monocytes and NRBC. The previously established Sysmex XT- 2000iV RIs can be used to interpret results from the Sysmex XN- V analyzer for most variables except red cell distribution width and reticulocyte variables. The RI for platelet variables could not be evaluated because of platelet clumps. Changes in the Sysmex XN- V measurements after storage at 4 and 24 degrees C were similar to those described for the Sysmex XT- 2000iV analyzer. Conclusions: The performance of the Sysmex XN- V analyzer was good and compared favorably with the Sysmex XT- 2000iV analyzer.