Genome-Wide Identification and Characterization of Ammonium Transporter (AMT) Genes in Chlamydomonas reinhardtii

被引:1
|
作者
Hu, Wenhui [1 ]
Wang, Dan [1 ]
Zhao, Shuangshuang [1 ]
Ji, Jiaqi [1 ]
Yang, Jing [1 ]
Wan, Yiqin [2 ]
Yu, Chao [1 ]
机构
[1] Nanchang Univ, Sch Life Sci, Nanchang 330031, Jiangxi, Peoples R China
[2] Nanchang Univ, Basic Expt Ctr Biol, Nanchang 330031, Jiangxi, Peoples R China
关键词
Chlamydomonas reinhardti; CrAMTs; ammonium transporters; bioinformatics; AMT1; family; AFFINITY NH4+ TRANSPORTER; PLASMA-MEMBRANE; RICE GENOTYPES; ARABIDOPSIS; NITROGEN; EXPRESSION; TOXICITY; MECHANISMS; INTRONS; NITRATE;
D O I
10.3390/genes15081002
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Ammonium transporters (AMTs) are vital plasma membrane proteins facilitating NH4+ uptake and transport, crucial for plant growth. The identification of favorable AMT genes is the main goal of improving ammonium-tolerant algas. However, there have been no reports on the systematic identification and expression analysis of Chlamydomonas reinhardtii (C. reinhardtii) AMT genes. This study comprehensively identified eight CrAMT genes, distributed across eight chromosomes, all containing more than 10 transmembrane structures. Phylogenetic analysis revealed that all CrAMTs belonged to the AMT1 subfamily. The conserved motifs and domains of CrAMTs were similar to those of the AMT1 members of OsAMTs and AtAMTs. Notably, the gene fragments of CrAMTs are longer and contain more introns compared to those of AtAMTs and OsAMTs. And the promoter regions of CrAMTs are enriched with cis-elements associated with plant hormones and light response. Under NH4+ treatment, CrAMT1;1 and CrAMT1;3 were significantly upregulated, while CrAMT1;2, CrAMT1;4, and CrAMT1;6 saw a notable decrease. CrAMT1;7 and CrAMT1;8 also experienced a decline, albeit less pronounced. Transgenic algas with overexpressed CrAMT1;7 did not show a significant difference in growth compared to CC-125, while transgenic algas with CrAMT1;7 knockdown exhibited growth inhibition. Transgenic algas with overexpressed or knocked-down CrAMT1;8 displayed reduced growth compared to CC-125, which also resulted in the suppression of other CrAMT genes. None of the transgenic algas showed better growth than CC-125 at high ammonium levels. In summary, our study has unveiled the potential role of CrAMT genes in high-ammonium environments and can serve as a foundational research platform for investigating ammonium-tolerant algal species.
引用
收藏
页数:20
相关论文
共 50 条
  • [41] Genome-Wide Identification and Characterization of MicroRNAs and Target Genes in Lonicera japonica
    Xia, Heng
    Zhang, Libin
    Wu, Gang
    Fu, Chunhua
    Long, Yan
    Xiang, Jun
    Gan, Jianping
    Zhou, Yanhong
    Yu, Longjiang
    Li, Maoteng
    PLOS ONE, 2016, 11 (10):
  • [42] Genome-Wide Identification and Characterization of RBR Ubiquitin Ligase Genes in Soybean
    Chen, Pei
    Zhang, Xiaolian
    Zhao, Tuanjie
    Li, Yan
    Gai, Junyi
    PLOS ONE, 2014, 9 (01):
  • [43] Genome-wide identification and characterization of GRAS genes in soybean (Glycine max)
    Liang Wang
    Xianlong Ding
    Yingqi Gao
    Shouping Yang
    BMC Plant Biology, 20
  • [44] Genome-wide identification and characterization of Fox genes in the silkworm, Bombyx mori
    JiangBo Song
    ZhiQuan Li
    XiaoLing Tong
    Cong Chen
    Min Chen
    Gang Meng
    Peng Chen
    ChunLin Li
    YaQun Xin
    TingTing Gai
    FangYin Dai
    Cheng Lu
    Functional & Integrative Genomics, 2015, 15 : 511 - 522
  • [45] Genome-Wide Identification and Characterization of Carboxypeptidase Genes in Silkworm (Bombyx mori)
    Ye, Junhong
    Li, Yi
    Liu, Hua-Wei
    Li, Jifu
    Dong, Zhaoming
    Xia, Qingyou
    Zhao, Ping
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2016, 17 (08)
  • [46] Genome-Wide Identification and Characterization of SPL Family Genes in Chenopodium quinoa
    Zhao, Hongmei
    Cao, Huaqi
    Zhang, Mian
    Deng, Sufang
    Li, Tingting
    Xing, Shuping
    GENES, 2022, 13 (08)
  • [47] Genome-wide identification and molecular characterization of cysteine protease genes in rice
    Nino, Marjohn C.
    Kim, Me-Sun
    Kang, Kwon Kyoo
    Cho, Yong-Gu
    PLANT BIOTECHNOLOGY REPORTS, 2020, 14 (01) : 69 - 87
  • [48] Genome-wide identification and characterization of genes involved in the acylsugar pathway in tomato
    Cardoso, Thais Cunha de Sousa
    Caneschi, Carolina Milagres
    Fernandes-Brum, Christiane Noronha
    Daude, Matheus Martins
    Ribeiro, Thales Henrique Cherubino
    Dos Reis, Gabriel Lasmar
    Lima, Andre Almeida
    Teixeira, Terezinha Aparecida
    Guimara, Ana Clara Garcia
    Gandolfi, Peterson Elizandro
    Gomes, Marcos de Souza
    do Amaral, Laurence Rodrigues
    Gomes, Luiz Antonio Augusto
    Chalfun-Junior, Antonio
    Maluf, Wilson Roberto
    Gomes, Matheus de Souza
    PLANT GENE, 2021, 28
  • [49] Genome-wide identification and characterization of maize expansin genes expressed in endosperm
    Wei Zhang
    Hanwei Yan
    Weijun Chen
    Jinyang Liu
    Cuiping Jiang
    Haiyang Jiang
    Suwen Zhu
    Beijiu Cheng
    Molecular Genetics and Genomics, 2014, 289 : 1061 - 1074
  • [50] Genome-wide identification and characterization of terpene synthase genes in Gossypium hirsutum
    Zhang, Cui-Ping
    Zhang, Jin-Li
    Sun, Zheng-Ran
    Liu, Xiu-Yan
    Shu, Li-Zhe
    Wu, Hao
    Song, Yin
    He, Dao-Hua
    GENE, 2022, 828