Ammonium transporters (AMTs) are vital plasma membrane proteins facilitating NH4+ uptake and transport, crucial for plant growth. The identification of favorable AMT genes is the main goal of improving ammonium-tolerant algas. However, there have been no reports on the systematic identification and expression analysis of Chlamydomonas reinhardtii (C. reinhardtii) AMT genes. This study comprehensively identified eight CrAMT genes, distributed across eight chromosomes, all containing more than 10 transmembrane structures. Phylogenetic analysis revealed that all CrAMTs belonged to the AMT1 subfamily. The conserved motifs and domains of CrAMTs were similar to those of the AMT1 members of OsAMTs and AtAMTs. Notably, the gene fragments of CrAMTs are longer and contain more introns compared to those of AtAMTs and OsAMTs. And the promoter regions of CrAMTs are enriched with cis-elements associated with plant hormones and light response. Under NH4+ treatment, CrAMT1;1 and CrAMT1;3 were significantly upregulated, while CrAMT1;2, CrAMT1;4, and CrAMT1;6 saw a notable decrease. CrAMT1;7 and CrAMT1;8 also experienced a decline, albeit less pronounced. Transgenic algas with overexpressed CrAMT1;7 did not show a significant difference in growth compared to CC-125, while transgenic algas with CrAMT1;7 knockdown exhibited growth inhibition. Transgenic algas with overexpressed or knocked-down CrAMT1;8 displayed reduced growth compared to CC-125, which also resulted in the suppression of other CrAMT genes. None of the transgenic algas showed better growth than CC-125 at high ammonium levels. In summary, our study has unveiled the potential role of CrAMT genes in high-ammonium environments and can serve as a foundational research platform for investigating ammonium-tolerant algal species.
机构:
CNTC, China Tobacco Gene Res Ctr, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Peoples R ChinaCNTC, China Tobacco Gene Res Ctr, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Peoples R China
Xu, Xin
Zeng, Wanli
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Yunnan China Tobacco Ind Co, Technol Ctr, Kunming 650000, Peoples R ChinaCNTC, China Tobacco Gene Res Ctr, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Peoples R China
Zeng, Wanli
Li, Zefeng
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CNTC, China Tobacco Gene Res Ctr, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Peoples R ChinaCNTC, China Tobacco Gene Res Ctr, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Peoples R China
Li, Zefeng
Wang, Zhong
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CNTC, China Tobacco Gene Res Ctr, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Peoples R ChinaCNTC, China Tobacco Gene Res Ctr, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Peoples R China
Wang, Zhong
Luo, Zhaopeng
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CNTC, China Tobacco Gene Res Ctr, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Peoples R ChinaCNTC, China Tobacco Gene Res Ctr, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Peoples R China
Luo, Zhaopeng
Li, Jing
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Yunnan China Tobacco Ind Co, Technol Ctr, Kunming 650000, Peoples R ChinaCNTC, China Tobacco Gene Res Ctr, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Peoples R China
Li, Jing
Li, Xuemei
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Yunnan China Tobacco Ind Co, Technol Ctr, Kunming 650000, Peoples R ChinaCNTC, China Tobacco Gene Res Ctr, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Peoples R China
Li, Xuemei
Yang, Jun
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CNTC, China Tobacco Gene Res Ctr, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Peoples R ChinaCNTC, China Tobacco Gene Res Ctr, Zhengzhou Tobacco Res Inst, Zhengzhou 450001, Peoples R China
机构:
Wuhan Univ, State Key Lab Hybrid Rice, Coll Life Sci, Wuhan 430072, Hubei, Peoples R ChinaWuhan Univ, State Key Lab Hybrid Rice, Coll Life Sci, Wuhan 430072, Hubei, Peoples R China
Li, Na
Wang, Yang
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Wuhan Univ, State Key Lab Hybrid Rice, Coll Life Sci, Wuhan 430072, Hubei, Peoples R ChinaWuhan Univ, State Key Lab Hybrid Rice, Coll Life Sci, Wuhan 430072, Hubei, Peoples R China
Wang, Yang
Lu, Jing
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Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R ChinaWuhan Univ, State Key Lab Hybrid Rice, Coll Life Sci, Wuhan 430072, Hubei, Peoples R China
Lu, Jing
Liu, Chuan
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Wuhan Univ, State Key Lab Hybrid Rice, Coll Life Sci, Wuhan 430072, Hubei, Peoples R China
Chongqing Univ Posts & Telecommun, Chongqing Key Lab Big Data Bio Intelligence, Chongqing 400065, Peoples R ChinaWuhan Univ, State Key Lab Hybrid Rice, Coll Life Sci, Wuhan 430072, Hubei, Peoples R China
机构:
State Key Lab Crop Biol, Tai An, Shandong, Peoples R China
Key Lab Biol & Genet Improvement Hort Crops Huang, Tai An, Shandong, Peoples R China
Shandong Collaborat Innovat Ctr Fruit & Vegetable, Tai An, Shandong, Peoples R China
Shandong Agr Univ, Coll Hort Sci & Engn, Tai An, Shandong, Peoples R ChinaState Key Lab Crop Biol, Tai An, Shandong, Peoples R China
Han, Ni
Tang, Rui
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State Key Lab Crop Biol, Tai An, Shandong, Peoples R China
Key Lab Biol & Genet Improvement Hort Crops Huang, Tai An, Shandong, Peoples R China
Shandong Collaborat Innovat Ctr Fruit & Vegetable, Tai An, Shandong, Peoples R China
Shandong Agr Univ, Coll Hort Sci & Engn, Tai An, Shandong, Peoples R ChinaState Key Lab Crop Biol, Tai An, Shandong, Peoples R China
Tang, Rui
Chen, Xueqian
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State Key Lab Crop Biol, Tai An, Shandong, Peoples R China
Key Lab Biol & Genet Improvement Hort Crops Huang, Tai An, Shandong, Peoples R China
Shandong Collaborat Innovat Ctr Fruit & Vegetable, Tai An, Shandong, Peoples R China
Shandong Agr Univ, Coll Hort Sci & Engn, Tai An, Shandong, Peoples R ChinaState Key Lab Crop Biol, Tai An, Shandong, Peoples R China
Chen, Xueqian
Xu, Zhixuan
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State Key Lab Crop Biol, Tai An, Shandong, Peoples R China
Key Lab Biol & Genet Improvement Hort Crops Huang, Tai An, Shandong, Peoples R China
Shandong Collaborat Innovat Ctr Fruit & Vegetable, Tai An, Shandong, Peoples R China
Shandong Agr Univ, Coll Hort Sci & Engn, Tai An, Shandong, Peoples R ChinaState Key Lab Crop Biol, Tai An, Shandong, Peoples R China
Xu, Zhixuan
Ren, Zhonghai
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State Key Lab Crop Biol, Tai An, Shandong, Peoples R China
Key Lab Biol & Genet Improvement Hort Crops Huang, Tai An, Shandong, Peoples R China
Shandong Collaborat Innovat Ctr Fruit & Vegetable, Tai An, Shandong, Peoples R China
Shandong Agr Univ, Coll Hort Sci & Engn, Tai An, Shandong, Peoples R ChinaState Key Lab Crop Biol, Tai An, Shandong, Peoples R China
Ren, Zhonghai
Wang, Lina
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State Key Lab Crop Biol, Tai An, Shandong, Peoples R China
Key Lab Biol & Genet Improvement Hort Crops Huang, Tai An, Shandong, Peoples R China
Shandong Collaborat Innovat Ctr Fruit & Vegetable, Tai An, Shandong, Peoples R China
Shandong Agr Univ, Coll Hort Sci & Engn, Tai An, Shandong, Peoples R ChinaState Key Lab Crop Biol, Tai An, Shandong, Peoples R China