Femtosecond fiber delivery at 920 nm for two-photon microscopy

被引：0
|
作者：
Birkmeier, Konrad [1 ]
Li, Yik Long [1 ]
Roeser, Fabian [1 ]
Herda, Robert [1 ]
Lohr, Laura [1 ]
Hofmann, Luisa [1 ]
Wolfring, Bernhard [1 ]
Haering, Reto [1 ]
Tauser, Florian [1 ]
机构：
[1] TOPTICA Photon AG, Lochhamer Schlag 19, D-82166 Graefelfing, Germany
来源：
MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XXIV | 2024年 / 12847卷
关键词：
Femtosecond laser; multi-photon microscopy; two-photon microscopy; ultrafast fiber delivery; LASER;
D O I：
10.1117/12.3002446
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
We present a femtosecond laser system at 920 nm delivering ultrashort pulses via a hollow-core photonic bandgap fiber (HC-PBGF). The laser system is designed to simplify two-photon microscopy applications and can be used for miniaturized two-photon microscopes.1 While previously presented solutions have been tailored to a specific length and dispersion coefficient of the HC-PBGF,1 we now show a compact and flexible scheme for dispersion compensation which is compatible with a wide range of fiber types and lengths. In addition, this new approach fully maintains the capability of software-controlled dispersion compensation in the range from 0 to -40,000 fs(2) after the pulse delivery fiber. Hence, the dispersion of common two-photon microscopes can be pre-compensated in order to obtain compressed pulses at the sample plane. Our newly developed system displays excellent long-term fiber coupling stability under varying environmental conditions. It is capable of polarization-preserving femtosecond pulse delivery at 920 nm and reaches Watt-level power after the delivery fiber, making it suitable for in-vivo brain imaging of GCaMP in mouse models.
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页数：8
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