Multifarious Two-Photon Fluorescence Microscopy Employing Ultrabroadband Femtosecond Laser Pulses

被引：0

作者：

Isobe, Keisuke ^{[1
]}

Suda, Akira ^{[1
]}

Hashimoto, Hiroshi ^{[2
,3
]}

Kannari, Fumihiko ^{[2
,3
]}

Kawano, Hiroyuki ^{[3
]}

Mizuno, Hideaki

Miyawaki, Atsushi

Midorikawa, Katsumi ^{[1
]}

机构：

[1] RIKEN, Adv Sci Inst, Laser Technol Lab, 2-1 Hirosawa, Wako, Saitama 3510198, Japan

[2] Keio Univ, Dept Elect & Elect Engn, Yokohama, Kanagawa 2238522, Japan

[3] RIKEN Brain Sci Institute, Lab Cell Funct Dynam, Wako, Saitama 3510198, Japan

来源：

FRONTIERS IN PHYSICS-BOOK | 2009年 / 1150卷

关键词：

Fluorescence; Biomedical imaging; Multiphoton processes; MULTIPHOTON INTRAPULSE INTERFERENCE; EXCITATION; PROTEINS; INDICATORS; CA2+;

D O I：

暂无

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

We demonstrate two-photon excited fluorescence (TPEF) microscopy employing the novel spectral phase modulation, which permits not only the selective excitation but also the simultaneous excitation together with controlling TPEF intensities from various fluorophores freely. The selective excitation is applied to the sequential dual-color imaging without emission crosstalk and FRET imaging of a HeLa cell. By the simultaneous excitation, dual-color imaging with various signal ratios including equal signal ratio is successfully achieved.

引用

页码：43 / +

页数：3

共 50 条

[1] High Speed 3D Two-photon Fluorescence Microscopy by Femtosecond Laser Pulses
Wang, Shixin
Qin, Yifan
Guo, Meishan
Zhang, Sheng
Xia, Yuanqin
LIDAR IMAGING DETECTION AND TARGET RECOGNITION 2017, 2017, 10605
[2] Two-Photon Laser Induced Fluorescence of Krypton Using Femtosecond Pulses
Wang, Yejun
Kulatilaka, Waruna D.
2017 CONFERENCE ON LASERS AND ELECTRO-OPTICS (CLEO), 2017,
[3] Polarized Fluorescence in Tryptophan Excited by Two-Photon Femtosecond Laser Pulses
Sasin, M. E.
Smolin, A. G.
Vasyutinskii, O. S.
OPTICS AND SPECTROSCOPY, 2018, 125 (04) : 516 - 521
[4] Polarized Fluorescence in Tryptophan Excited by Two-Photon Femtosecond Laser Pulses
M. E. Sasin
A. G. Smolin
O. S. Vasyutinskii
Optics and Spectroscopy, 2018, 125 : 516 - 521
[5] Construction and application of femtosecond laser two-photon fluorescence microscopy system
Qin, Yifan
Li, Qian
Xia, Yuanqin
Liu, Bin
Zhang, Sheng
Harbin Gongye Daxue Xuebao/Journal of Harbin Institute of Technology, 2015, 47 (11): : 1 - 5
[6] Selective two-photon microscopy with shaped femtosecond pulses
Pastirk, I
Dela Cruz, JM
Walowicz, KA
Lozovoy, VV
Dantus, M
OPTICS EXPRESS, 2003, 11 (14): : 1695 - 1701
[7] Coherent control of two-photon excited fluorescence with ultrabroadband optical pulses
Tanaka, Masahiro
Isobe, Keisuke
Suda, Akira
Kannari, Fumihiko
Kawan, Hiroyuki
Mizuno, Hideaki
Miyawaki, Atsushi
Midorikawa, Katsumi
2008 CONFERENCE ON LASERS AND ELECTRO-OPTICS & QUANTUM ELECTRONICS AND LASER SCIENCE CONFERENCE, VOLS 1-9, 2008, : 2697 - +
[8] Polarized fluorescence in NADH under two-photon excitation with femtosecond laser pulses
Vasyutinskii, O. S.
Smolin, A. G.
Oswald, C.
Gericke, K. H.
OPTICS AND SPECTROSCOPY, 2017, 122 (04) : 602 - 606
[9] Polarized fluorescence in NADH under two-photon excitation with femtosecond laser pulses
O. S. Vasyutinskii
A. G. Smolin
C. Oswald
K. H. Gericke
Optics and Spectroscopy, 2017, 122 : 602 - 606
[10] Polarized Fluorescence in Indole under Two-Photon Excitation by Femtosecond Laser Pulses
M. E. Sasin
V. I. Tushkanov
A. G. Smolin
P. S. Shternin
O. S. Vasyutinskii
Technical Physics, 2018, 63 : 1293 - 1299

← 1 2 3 4 5 →