The interplay of growth-regulating factor 5 and BZR1 in coregulating chlorophyll degradation in poplar

被引：4
|
作者：
Chen, Hao [1 ,2 ]
Wu, Wenqi [3 ]
Du, Kang [1 ,2 ]
Ling, Aoyu [1 ,2 ]
Kang, Xiangyang [1 ,2 ]
机构：
[1] Beijing Forestry Univ, Coll Biol Sci & Technol, State Key Lab Tree Genet & Breeding, Beijing 100083, Peoples R China
[2] Beijing Forestry Univ, Coll Biol Sci & Technol, Natl Engn Res Ctr Tree Breeding & Ecol Restorat, Beijing, Peoples R China
[3] Chinese Acad Agr Sci, Inst Crop Sci, Beijing, Peoples R China
来源：
PLANT CELL AND ENVIRONMENT | 2024年 / 47卷 / 10期
基金：
国家重点研发计划;
关键词：
brassinosteroid; PpnGRF5-1; INDUCED LEAF SENESCENCE; TRANSCRIPTION FACTORS; HORMONAL-REGULATION; GENE-EXPRESSION; PLANT-GROWTH; BRASSINOSTEROIDS; INTEGRATION; EFFICIENCY; DAMAGE; ROLES;
D O I：
10.1111/pce.14958
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Chlorophyll (Chl) is essential for plants to carry out photosynthesis, growth and development processes. Growth-regulating factors (GRFs) play a vital role in regulating Chl degradation in plants. However, the molecular mechanism by which GRF5 regulates Chl degradation in poplar remains unknown. Here we found that overexpression of PpnGRF5-1 increased Chl content in leaves and promoted chloroplast development in poplar. Overexpression of PpnGRF5-1 in poplar delayed Chl degradation induced by external factors, such as hormones, darkness and salt stress. PpnGRF5-1 responded to brassinosteroid (BR) signalling during BR-induced Chl degradation and reduced the expression levels of Chl degradation and senescence-related genes. PpnGRF5-1 inhibited the expression of Chl b reductases PagNYC1 and PagNOL. PpnGRF5-1 could interact with PagBZR1 in the nucleus. PagBZR1 also inhibited the expression of PagNYC1. In addition, we found that the protein-protein interaction between PagBZR1 and PpnGRF5-1 enhanced the inhibitory effect of PpnGRF5-1 on the Chl b reductases PagNYC1 and PagNOL. BZR1 and GRF5-1 were upregulated, and NOL and NYC1 were downregulated in triploid poplars compared to diploids. This study revealed a new mechanism by which PpnGRF5-1 regulates Chl degradation in poplars and lays the foundation for comprehensively analysing the molecular mechanism of Chl metabolism in triploid poplars. PpnGRF5-1 could interact with PagBZR1 in the nucleus and bind to the promoter of the Chl b reductases PagNYC1 and PagNOL to regulate Chl degradation in poplar.
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页码：3766 / 3779
页数：14
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