Deep-UV fluorescence lifetime imaging microscopy

被引:0
|
作者
Christiaan Jde Jong [1 ,2 ]
Alireza Lajevardipour [1 ]
Mindaugas Geceviius [3 ]
Martynas Beresna [3 ]
Gediminas Gervinskas [1 ,4 ]
Peter GKazansky [3 ]
Yves Bellouard [5 ]
Andrew HAClayton [1 ]
Saulius Juodkazis [1 ,4 ,6 ]
机构
[1] Centre for Micro-Photonics, Faculty of Science, Engineering and Technology, Swinburne University of Technology
[2] Department of Mechanical Engineering, Eindhoven University of Technology
[3] Optoelectronics Research Centre, University of Southampton
[4] Melbourne Centre for Nanofabrication
[5] Ecole Polytechnique Fédérale de Lausanne, Rue de la Maladiére b
[6] Center for Nanotechnology, King Abdulaziz
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TH742 [显微镜];
学科分类号
摘要
A novel fluorescence lifetime imaging microscopy(FLIM) working with deep UV 240–280 nm wavelength excitations has been developed. UV-FLIM is used for measurement of defect-related fluorescence and its changes upon annealing from femtosecond laser-induced modifications in fused silica. This FLIM technique can be used with microfluidic and biosamples to characterize temporal characteristics of fluorescence upon UV excitation, a capability easily added to a standard microscope-based FLIM. UV-FLIM was tested to show annealing of the defects induced by silica structuring with ultrashort laser pulses. Frequency-domain fluorescence measurements were converted into the time domain to extract long fluorescence lifetimes from defects in silica.
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页码:283 / 288
页数:6
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