Production and Optimization of Cellulase Enzymes from Newly Isolated Fungi

The main purpose of this study to reduced the production cost of cellulase by using alternative carbon source such as agricultural waste residue and optimized fermentation medium for high yielding. The present investigation was carried out to isolate the novel cellulase producing fungi, CPF-1 & CPF-2 with optimization of physicochemical and nutritional parameters for cellulase production. Among agricultural residues, rice bran and wheat straw were found to be best carbon source for the production of cellulase by CPF-1 and CPF-2, respectively. The optimal parameter (Temp, pH and incubation time) for the production of cellulase by CPF-1 and CPF-2 were 28 degrees C, pH 6 & 120 min and 28 degrees C, pH 7 & 96 min respectively. Also the thermal stability of both the purified cellulase enzymes was high at 60 C for 85min & 70 min for CPF-1 and CPF-2 fungi respectively.