Production and Optimization of Cellulase Enzymes from Newly Isolated Fungi

被引：0
|
作者：
Patel, Khushbu [1 ]
Patel, Hiren [2 ]
Shah, Gaurav [1 ]
机构：
[1] Veer Narmad South Gujarat Univ, Dept Biotechnol, Surat 395007, India
[2] Navsari Agr Univ, Dept Plant Mol Biol & Biotechnol, Navsari 396450, India
来源：
JOURNAL OF PURE AND APPLIED MICROBIOLOGY | 2014年 / 8卷 / 05期
关键词：
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The main purpose of this study to reduced the production cost of cellulase by using alternative carbon source such as agricultural waste residue and optimized fermentation medium for high yielding. The present investigation was carried out to isolate the novel cellulase producing fungi, CPF-1 & CPF-2 with optimization of physicochemical and nutritional parameters for cellulase production. Among agricultural residues, rice bran and wheat straw were found to be best carbon source for the production of cellulase by CPF-1 and CPF-2, respectively. The optimal parameter (Temp, pH and incubation time) for the production of cellulase by CPF-1 and CPF-2 were 28 degrees C, pH 6 & 120 min and 28 degrees C, pH 7 & 96 min respectively. Also the thermal stability of both the purified cellulase enzymes was high at 60 C for 85min & 70 min for CPF-1 and CPF-2 fungi respectively.
引用
收藏
页码：4163 / 4169
页数：7
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