COMPARISON OF METHODS USED FOR DETECTION OF MYCOPLASMA CONTAMINATION IN CELL-CULTURES, SERA, AND LIVE-VIRUS VACCINES

被引：0
|
作者：
BENISHEVA, T
SOVOVA, V
IVANOV, I
OPALCHENOVA, G
机构：
[1] ACAD SCI CZECH REPUBL, INST MOLEC GENET, CS-16637 PRAGUE, CZECHOSLOVAKIA
[2] BULGARIAN ACAD SCI, INST GEN & COMPARAT PATHOL, BU-1113 SOFIA, BULGARIA
来源：
FOLIA BIOLOGICA | 1993年 / 39卷 / 05期
关键词：
MYCOPLASMA; DETECTION TECHNIQUES; CONTAMINATION OF CELL CULTURE;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two methods for detection of mycoplasma contamination in cell cultures, sera, and live-virus vaccines were compared: the direct culture test and the DNA staining method employing bisBenzimide (Hoechst No. 33258). Contamination by different species of mycoplasma was found in 39% samples tested. It is recommended to use both techniques for a reliable detection of mycoplasma contamination.
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页码：270 / 275
页数：6
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