COMPARISON OF METHODS USED FOR DETECTION OF MYCOPLASMA CONTAMINATION IN CELL-CULTURES, SERA, AND LIVE-VIRUS VACCINES

被引：0

作者：

BENISHEVA, T

SOVOVA, V

IVANOV, I

OPALCHENOVA, G

机构：

[1] ACAD SCI CZECH REPUBL, INST MOLEC GENET, CS-16637 PRAGUE, CZECHOSLOVAKIA

[2] BULGARIAN ACAD SCI, INST GEN & COMPARAT PATHOL, BU-1113 SOFIA, BULGARIA

来源：

FOLIA BIOLOGICA | 1993年 / 39卷 / 05期

关键词：

MYCOPLASMA; DETECTION TECHNIQUES; CONTAMINATION OF CELL CULTURE;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two methods for detection of mycoplasma contamination in cell cultures, sera, and live-virus vaccines were compared: the direct culture test and the DNA staining method employing bisBenzimide (Hoechst No. 33258). Contamination by different species of mycoplasma was found in 39% samples tested. It is recommended to use both techniques for a reliable detection of mycoplasma contamination.

引用

页码：270 / 275

页数：6

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