COMPLEMENTARY-DNA CLONING AND NUCLEOTIDE-SEQUENCE OF RABBIT SERUM AMYLOID A PROTEIN

A complementary DNA clone encoding serum amyloid A protein has been isolated from an acute rabbit liver cDNA library. Complete nucleotide sequence analysis reveals that the cloned gene contains a 24 bases 5′ untranslated region, 369 bases coding region and a 106 bases 3′ untraslated region. Primer extension analysis indicates that the full-length 5′ untranslated region contains 80 nucleotides. Northern blot analysis of mRNA from normal and acute rabbit livers demonstrates that this gene is expressed constitutively at a low level and undergoes induction of transcription in response to acute inflammation by the administration of turpentine. © 1991.