PARTITIONING OF YEAST RNA IN AQUEOUS TWO-PHASE SYSTEM

被引：1
|
作者：
Kimura, K. [1 ]
Kobayashi, H. [1 ]
机构：
[1] Fermentat Res Inst, Tsukuba, Ibaraki 305, Japan
来源：
BIOTECHNOLOGY TECHNIQUES | 1992年 / 6卷 / 02期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The partition behaviour of RNA extracted from yeast was examined using three types of aqueous two-phase systems: potassium phosphate-polyethyleneglycol (PEG) system, ammonium sulphate-PEG system and dextran sulphate-PEG-NaCl system. The molecular weight of PEG showed rather remarkable effects on RNA partition than did the electrolyte. High molecular weight RNA was concentrated at the interface in these systems. This suggests the possibility of its simple concentration or extraction.
引用
收藏
页码：149 / 154
页数：6
相关论文
共 50 条
  • [41] Affinity partitioning of human antibodies in aqueous two-phase systems
    Rosa, P. A. J.
    Azevedo, A. M.
    Ferreira, I. F.
    de Vries, J.
    Korporaal, R.
    Verhoef, H. J.
    Visser, T. J.
    Aires-Barros, M. R.
    JOURNAL OF CHROMATOGRAPHY A, 2007, 1162 (01) : 103 - 113
  • [42] Quantitative prediction of phase diagrams for polymer partitioning in aqueous two-phase systems
    Croll, T
    Munro, PD
    Winzor, DJ
    Trau, M
    Nielsen, LK
    JOURNAL OF POLYMER SCIENCE PART B-POLYMER PHYSICS, 2003, 41 (05) : 437 - 443
  • [43] Partitioning of cephalosporins in acetone/NaCl two-phase system
    Zhou, Xu
    Zhang, Yan
    Zhao, Fengsheng
    FLUID PHASE EQUILIBRIA, 2012, 319 : 1 - 4
  • [44] Improved partitioning in aqueous two-phase system of tyrosine-tagged recombinant lactate dehydrogenase
    Fexby, S
    Bülow, L
    PROTEIN EXPRESSION AND PURIFICATION, 2002, 25 (02) : 263 - 269
  • [45] Partitioning of porphyrin compounds in an aqueous two-phase system of cationic-anionic surfactant mixture
    Tong, AJ
    Wu, Y
    Li, LD
    Akama, Y
    Tanaka, S
    ANALYTICAL SCIENCES, 1997, 13 : 111 - 114
  • [46] Partitioning and purification of lysozyme from chicken egg white using aqueous two-phase system
    Su, CK
    Chiang, BH
    PROCESS BIOCHEMISTRY, 2006, 41 (02) : 257 - 263
  • [47] Influence of system and process parameters on partitioning of cheese whey proteins in aqueous two-phase systems
    Rito-Palomares, M
    Hernandez, M
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1998, 711 (1-2): : 81 - 90
  • [48] Partitioning of Proteins using a Hydrophobically Modified Ethylene Oxide/SDS Aqueous Two-phase System
    Gang Deng
    Shan-Jing Yao
    Dong-Qiang Lin
    World Journal of Microbiology and Biotechnology, 2005, 21 : 1209 - 1214
  • [49] Influence of system and process parameters on partitioning of cheese whey proteins in aqueous two-phase systems
    Rito-Palomares, Marco
    Hernandez, Miguel
    Journal of Chromatography B, 1998, 711 (1-2): : 81 - 90
  • [50] Partitioning of proteins using a hydrophobically modified ethylene oxide/SDS aqueous two-phase system
    Deng, G
    Yao, SJ
    Lin, DQ
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2005, 21 (6-7): : 1209 - 1214
12345