PARTITIONING OF YEAST RNA IN AQUEOUS TWO-PHASE SYSTEM

被引：1
|
作者：
Kimura, K. [1 ]
Kobayashi, H. [1 ]
机构：
[1] Fermentat Res Inst, Tsukuba, Ibaraki 305, Japan
来源：
BIOTECHNOLOGY TECHNIQUES | 1992年 / 6卷 / 02期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The partition behaviour of RNA extracted from yeast was examined using three types of aqueous two-phase systems: potassium phosphate-polyethyleneglycol (PEG) system, ammonium sulphate-PEG system and dextran sulphate-PEG-NaCl system. The molecular weight of PEG showed rather remarkable effects on RNA partition than did the electrolyte. High molecular weight RNA was concentrated at the interface in these systems. This suggests the possibility of its simple concentration or extraction.
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页码：149 / 154
页数：6
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