DETECTION OF THE BOVINE HERPESVIRUS-1 (BHV-1) GENOME BY PCR

被引：54

作者：

VILCEK, S

机构：

[1] INST EXPTL VET MED, JOINT LAB GENET MANIPULAT, KOSICE, CZECHOSLOVAKIA

[2] UNIV VET MED, KOSICE, CZECHOSLOVAKIA

来源：

JOURNAL OF VIROLOGICAL METHODS | 1993年 / 41卷 / 02期

关键词：

BHV-1; PCR METHOD; DOT-BLOT HYBRIDIZATION;

D O I：

10.1016/0166-0934(93)90132-B

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The amplification of the 468 bp fragment of the BHV-1 genome by PCR is described. The 22 bp oligomers from the BHV-1 gI gene were used as primers. For successful amplification the thermal denaturation (100-degrees-C/8 min, ice) of the DNA sample was carried out prior to the cycling (95-degrees-C for 1 min, 56-degrees-C for 1 min, 73-degrees-C for 1 min). The presence of glycerol markedly enhances the PCR.

引用

页码：245 / 247

页数：3

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