DETECTION OF THE BOVINE HERPESVIRUS-1 (BHV-1) GENOME BY PCR

被引：54

作者：

VILCEK, S

机构：

[1] INST EXPTL VET MED, JOINT LAB GENET MANIPULAT, KOSICE, CZECHOSLOVAKIA

[2] UNIV VET MED, KOSICE, CZECHOSLOVAKIA

来源：

JOURNAL OF VIROLOGICAL METHODS | 1993年 / 41卷 / 02期

关键词：

BHV-1; PCR METHOD; DOT-BLOT HYBRIDIZATION;

D O I：

10.1016/0166-0934(93)90132-B

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The amplification of the 468 bp fragment of the BHV-1 genome by PCR is described. The 22 bp oligomers from the BHV-1 gI gene were used as primers. For successful amplification the thermal denaturation (100-degrees-C/8 min, ice) of the DNA sample was carried out prior to the cycling (95-degrees-C for 1 min, 56-degrees-C for 1 min, 73-degrees-C for 1 min). The presence of glycerol markedly enhances the PCR.

引用

页码：245 / 247

页数：3

共 50 条

[21] Epitopes on glycoprotein C of bovine herpesvirus-1 (BHV-1) that allow differentiation between BHV-1.1 and BHV-1.2 strains
Rijsewijk, FAM
Kaashoek, MJ
Langeveld, JPM
Meloen, R
Judek, J
Bienkowska-Szewczyk, K
Maris-Veldhuis, MA
van Oirschot, JT
JOURNAL OF GENERAL VIROLOGY, 1999, 80 : 1477 - 1483
[22] A retrospective evaluation of a Bovine Herpesvirus-1 (BHV-1) antibody ELISA on bulk-tank milk samples for classification of the BHV-1 status of Danish dairy herds
Nylin, B
Stroger, U
Ronsholt, L
PREVENTIVE VETERINARY MEDICINE, 2000, 47 (1-2) : 91 - 105
[23] A chimeric baculovirus displaying bovine herpesvirus-1 (BHV-1) glycoprotein D on its surface and their immunological properties
Peralta, A.
Molinari, P.
Conte-Grand, D.
Calamate, G.
Taboga, O.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2007, 75 (02) : 407 - 414
[24] A chimeric baculovirus displaying bovine herpesvirus-1 (BHV-1) glycoprotein D on its surface and their immunological properties
A. Peralta
P. Molinari
D. Conte-Grand
G. Calamante
O. Taboga
Applied Microbiology and Biotechnology, 2007, 75 : 407 - 414
[25] Bovine Herpesvirus Type 1 (BHV-1) Prevalence in Dairy Cattle
Iscan, Uzeyir Taha
Duman, Rustem
JOURNAL OF ANIMAL AND VETERINARY ADVANCES, 2011, 10 (12): : 1523 - 1525
[26] ISOLATION OF BOVINE HERPESVIRUS-1 (BHV-1) AND BOVINE VIRAL DIARRHEA VIRUS (BVDV) IN ASSOCIATION WITH THE IN-VITRO PRODUCTION OF BOVINE EMBRYOS
BIELANSKI, A
LOEWEN, KS
DELCAMPO, MR
SIRARD, MA
WILLADSEN, S
THERIOGENOLOGY, 1993, 40 (03) : 531 - 538
[27] THE GENOME OF CAPRINE HERPESVIRUS-1 - GENOME STRUCTURE AND RELATEDNESS TO BOVINE HERPESVIRUS-1
ENGELS, M
LOEPFE, E
WILD, P
SCHRANER, E
WYLER, R
JOURNAL OF GENERAL VIROLOGY, 1987, 68 : 2019 - 2023
[28] Development and viability of bovine preimplantation embryos after the in vitro infection with bovine herpesvirus-1 (BHV-1): immunocytochemical and ultrastructural studies
Makarevich, A. V.
Pivko, J.
Kubovicova, E.
Chrenek, P.
Slezakova, M.
Louda, F.
ZYGOTE, 2007, 15 (04) : 307 - 315
[29] Detection of BHV-1 in a naturally infected bovine fetus by a nested PCR assay
Rocha, MA
Barbosa, EF
Guedes, RMC
Lage, AP
Leite, RC
Gouveia, AMG
VETERINARY RESEARCH COMMUNICATIONS, 1999, 23 (02) : 133 - 141
[30] Detection of BHV-1 in a Naturally Infected Bovine Fetus by a Nested PCR assay
M.A. Rocha
E.F. Barbosa
R.M.C. Guedes
A.P. Lage
R.C. Leite
A.M.G. Gouveia
Veterinary Research Communications, 1999, 23 : 133 - 141

← 1 2 3 4 5 →