CHARACTERIZATION OF PROTEASES PRODUCED BY NEWLY ISOLATED AND IDENTIFIED PROTEOLYTIC MICROORGANISMS FROM FERMENTED FISH (BUDU)

被引:11
|
作者
CHOORIT, W [1 ]
PRASERTSAN, P [1 ]
机构
[1] PRINCE SONGKLA UNIV,FAC NAT RESOURCES,DEPT AGROIND,HAT YAI 90110,THAILAND
来源
关键词
BACILLUS; BUDU; FERMENTATION; FISH; PROTEASES;
D O I
10.1007/BF01201880
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Eight different strains of Bacillus were isolated from fermented fish (Budu) and their proteolytic enzyme activities were determined after 18 h cultivation at room temperature (35-degrees-C). Four isolates possessed high protease activities. Optimum pH for these enzymes was between 7.0 and 8.0 and the optimal temperature was 55-degrees-C. The proteases retained 40% of their original activity after 20 min at 55-degrees-C but lost all activity at 65-degrees-C. Three of the four isolates were identified as Bacillus subtilis, the fourth as Bacillus licheniformis.
引用
收藏
页码:284 / 286
页数:3
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