Eight different strains of Bacillus were isolated from fermented fish (Budu) and their proteolytic enzyme activities were determined after 18 h cultivation at room temperature (35-degrees-C). Four isolates possessed high protease activities. Optimum pH for these enzymes was between 7.0 and 8.0 and the optimal temperature was 55-degrees-C. The proteases retained 40% of their original activity after 20 min at 55-degrees-C but lost all activity at 65-degrees-C. Three of the four isolates were identified as Bacillus subtilis, the fourth as Bacillus licheniformis.
机构:
Univ Nacl Mar del Plata, Fac Ingn, Dept Ingn Quim, Grp Invest Ingn Alimentos, RA-7600 Buenos Aires, DF, Argentina
Consejo Nacl Invest Cient & Tecn, RA-1033 Buenos Aires, DF, ArgentinaUniv Nacl Mar del Plata, Fac Ingn, Dept Ingn Quim, Grp Invest Ingn Alimentos, RA-7600 Buenos Aires, DF, Argentina
Perez Borla, O.
Davidovich, L. A.
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机构:Univ Nacl Mar del Plata, Fac Ingn, Dept Ingn Quim, Grp Invest Ingn Alimentos, RA-7600 Buenos Aires, DF, Argentina
Davidovich, L. A.
Roura, S. I.
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Univ Nacl Mar del Plata, Fac Ingn, Dept Ingn Quim, Grp Invest Ingn Alimentos, RA-7600 Buenos Aires, DF, Argentina
Consejo Nacl Invest Cient & Tecn, RA-1033 Buenos Aires, DF, ArgentinaUniv Nacl Mar del Plata, Fac Ingn, Dept Ingn Quim, Grp Invest Ingn Alimentos, RA-7600 Buenos Aires, DF, Argentina
机构:
Andalas Univ, Dept Anim Nutr, Fac Anim Sci, Padang, West Sumatera, IndonesiaAndalas Univ, Fac Anim Sci, PMDSU Program, Grad Program, Padang, West Sumatera, Indonesia
Marlida, Yetti
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Zain, Mardiati
Jamsari, Jamsari
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Andalas Univ, Fac Agr, Dept Plant Breeding, Padang, West Sumatera, IndonesiaAndalas Univ, Fac Anim Sci, PMDSU Program, Grad Program, Padang, West Sumatera, Indonesia
Jamsari, Jamsari
Fassah, Dilla Mareistia
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IPB Univ, Fac Anim Sci, Dept Nutr & Feed Technol, Bogor, West Java, IndonesiaAndalas Univ, Fac Anim Sci, PMDSU Program, Grad Program, Padang, West Sumatera, Indonesia
机构:
Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Bioproc Technol, Upm Serdang 43400, Selangor, MalaysiaUniv Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Bioproc Technol, Upm Serdang 43400, Selangor, Malaysia
Abbasiliasi, S.
Ramanan, R. Nagasundara
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Univ Putra Malaysia, Inst Biosci, Serdang, Malaysia
Monash Univ, Chem & Sustainable Proc Engn Res Grp, Sch Engn, Bandar Sunway, MalaysiaUniv Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Bioproc Technol, Upm Serdang 43400, Selangor, Malaysia
Ramanan, R. Nagasundara
Ibrahim, T. Azmi Tengku
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Univ Putra Malaysia, Inst Biosci, Serdang, Malaysia
Univ Putra Malaysia, Dept Vet Preclin Sci, Fac Vet Med, Serdang, MalaysiaUniv Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Bioproc Technol, Upm Serdang 43400, Selangor, Malaysia
Ibrahim, T. Azmi Tengku
Shuhaimi, M.
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Univ Putra Malaysia, Dept Microbiol, Fac Biotechnol & Biomol Sci, Serdang, MalaysiaUniv Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Bioproc Technol, Upm Serdang 43400, Selangor, Malaysia
Shuhaimi, M.
Rosfarizan, M.
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Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Bioproc Technol, Upm Serdang 43400, Selangor, Malaysia
Univ Putra Malaysia, Inst Biosci, Serdang, MalaysiaUniv Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Bioproc Technol, Upm Serdang 43400, Selangor, Malaysia
Rosfarizan, M.
Ariff, A. B.
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Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Bioproc Technol, Upm Serdang 43400, Selangor, Malaysia
Univ Putra Malaysia, Inst Biosci, Serdang, MalaysiaUniv Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Bioproc Technol, Upm Serdang 43400, Selangor, Malaysia