DETECTION OF FOOT-AND-MOUTH-DISEASE VIRUS IN NASAL SWABS OF ASYMPTOMATIC CATTLE BY RT-PCR WITHIN 24 HOURS

被引：32

作者：

MARQUARDT, O

STRAUB, OC

AHL, R

HAAS, B

机构：

[1] Bundesforschungsanstalt für Viruskrankheiten der Tiere, Tubingen, D-72001

来源：

JOURNAL OF VIROLOGICAL METHODS | 1995年 / 53卷 / 2-3期

关键词：

FOOT-AND-MOUTH DISEASE (FMD); POLYMERASE CHAIN REACTION; NASAL SWAB; DIAGNOSIS;

D O I：

10.1016/0166-0934(95)00015-M

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for extracting RNA from animal-derived materials that provides foot-and-mouth disease viral template suitable for Tth polymerase-dependent synthesis of cDNA and subsequent PCR is described. Viral genomes were detected in less than 24 h. Nasal swabs that can be easily and repeatedly collected, proved suitable for virus detection by PCR, even during the asymptomatic stages of infection.

引用

页码：255 / 261

页数：7

共 50 条

[31] RAPID COAGGLUTINATION TEST FOR THE DETECTION AND TYPING OF FOOT-AND-MOUTH-DISEASE VIRUS
MONTASSIER, HJ
ARAUJO, JP
PINTO, AA
JOURNAL OF VIROLOGICAL METHODS, 1994, 50 (1-3) : 29 - 41
[32] A SENSITIVE METHOD FOR THE DETECTION AND ISOLATION OF RECOMBINANTS OF FOOT-AND-MOUTH-DISEASE VIRUS
MCCAHON, D
SLADE, WR
JOURNAL OF GENERAL VIROLOGY, 1981, 53 (APR): : 333 - 342
[33] Analysis of foot-and-mouth disease virus replication using strand-specific quantitative RT-PCR
Horsington, Jacquelyn
Zhang, Zhidong
JOURNAL OF VIROLOGICAL METHODS, 2007, 144 (1-2) : 149 - 155
[34] MULTIPLE SITES OF RECOMBINATION WITHIN THE RNA GENOME OF FOOT-AND-MOUTH-DISEASE VIRUS
KING, AMQ
MCCAHON, D
SAUNDERS, K
NEWMAN, JWI
SLADE, WR
VIRUS RESEARCH, 1985, 3 (04) : 373 - 384
[35] A Survey on the Frequency of Foot-and-Mouth Disease Virus Carriers in Cattle in North-East of Iran by RT-PCR: Implications for Revising Disease Control Strategy
Rashtibaf, M.
Sharifi, K.
Zibaee, S.
Dehghani, H.
TRANSBOUNDARY AND EMERGING DISEASES, 2012, 59 (06) : 482 - 489
[36] INVITRO COMPARISON OF FOOT-AND-MOUTH-DISEASE VIRUS SUBTYPE VARIANTS CAUSING DISEASE IN VACCINATED CATTLE
ANDERSON, EC
DOUGHTY, WJ
ANDERSON, J
BABER, D
JOURNAL OF HYGIENE, 1978, 80 (03) : 451 - 459
[37] BIOCHEMICAL-CHARACTERIZATION OF A FOOT-AND-MOUTH-DISEASE VIRUS-STRAIN ATTENUATED FOR CATTLE
POLACINO, P
KAPLAN, G
YAFAL, AG
PALMA, EL
ARCHIVES OF VIROLOGY, 1986, 88 (1-2) : 143 - 150
[38] GENETIC-VARIATION OF FOOT-AND-MOUTH-DISEASE VIRUS DURING PERSISTENT INFECTION IN CATTLE
MALIRAT, V
DEMELLO, PA
TIRABOSCHI, B
BECK, E
GOMES, I
BERGMANN, IE
VIRUS RESEARCH, 1994, 34 (01) : 31 - 48
[39] DETECTION OF FOOT-AND-MOUTH-DISEASE VIRUS-INFECTED CATTLE BY ASSESSMENT OF ANTIBODY-RESPONSE IN OROPHARYNGEAL FLUIDS
ARCHETTI, IL
AMADORI, M
DONN, A
SALT, J
LODETTI, E
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (01) : 79 - 84
[40] SURVIVAL OF FOOT-AND-MOUTH-DISEASE VIRUS IN AFRICAN BUFFALO WITH NONTRANSFERENCE OF INFECTION TO DOMESTIC CATTLE
CONDY, JB
HEDGER, RS
RESEARCH IN VETERINARY SCIENCE, 1974, 16 (02) : 182 - 185

← 1 2 3 4 5 →