INVITRO INTERACTION BETWEEN CEREBELLAR ASTROCYTES AND GRANULE CELLS - A PUTATIVE ROLE FOR NITRIC-OXIDE

被引:47
|
作者
KIEDROWSKI, L [1 ]
COSTA, E [1 ]
WROBLEWSKI, JT [1 ]
机构
[1] GEORGETOWN UNIV,SCH MED,FIDIA GEORGETOWN INST NEUROSCI,3900 RESERVOIR RD NW,WASHINGTON,DC 20007
关键词
CEREBELLAR GRANULE CELL; ASTROCYTE; N-METHYL-D-ASPARTATE; CYCLIC GUANOSINE MONOPHOSPHATE; CA2+ INFLUX; L-CITRULLINE; NITRIC OXIDE;
D O I
10.1016/0304-3940(92)90135-T
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
In primary cultures of astrocytes and granule cells from neonatal rat cerebellum, the activity and function of nitric oxide (NO) synthase were measured by the conversion of [H-3]arginine to [H-3]citrulline and the accumulation of cyclic guanosine monophosphate (cGMP), respectively. The glutamate receptor agonist N-methyl-D-aspartate (NMDA) and the Ca2+ ionophore A23187 stimulated NO synthase activity in cerebellar granule cells but not in astrocytes. In granule cells, NMDA, A23187, and sodium nitroprusside (SNP) elicited an accumulation of cGMP, whereas only SNP was active in astrocytes. However, in astrocytes that were incubated together with granule cells, NMDA induced a more than 3-fold increase in the concentration of cGMP; this increase was blocked by both the NO synthase inhibitor N(G)-monomethyl-L-arginine (MeArg) and the allosteric NMDA receptor antagonist (+)5-methyl-10,11-dihydro-5H-dibenzocyclohepten-5,10-imine maleate (MK-801). Thus, cerebellar astrocytes do not appear to express NO synthase but do contain guanylate cyclase, which can be activated by an NO-like factor produced by cerebellar granule cells after stimulation by NMDA.
引用
收藏
页码:59 / 61
页数:3
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