Nylon filters, a new technique for the pancreas islet isolation in a canine research model, a technical standardization

被引：0

作者：

Bernal, Jaime ^{[1
]}

Martinez, Fernando ^{[1
]}

Henao, Francisco ^{[2
]}

Pulido, Hector

Mejia, Gilberto

Benavides, Carlos ^{[2
]}

Jaime Echeverri, Gabriel ^{[2
]}

Maria Uribe, Ana ^{[3
]}

Danobeitia, Sebastian ^{[1
]}

机构：

[1] Pontificia Univ Javeriana, Inst Genet Humana, Bogota, DC, Colombia

[2] Hosp Univ San Ignacio, Dept Cirug, Bogota, DC, Colombia

[3] Hosp Univ San Ignacio, Dept Patol, Bogota, DC, Colombia

来源：

UNIVERSITAS MEDICA | 2009年 / 50卷 / 04期

关键词：

Diabetes Mellitus; transplantation;

D O I：

暂无

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Pancreas islet transplantation is a developing field for the treatment of diabetes mellitus. Currently the isolation technique is a complex process that still has some problems to overcome. Two of the problems we addressed were the high cost of the procedure and the cellular toxicity derived from Ficoll use during purification of pancreatic islets of Langerhans during the isolation procedure. We focused our efforts in reducing both the cost of the procedure and cellular injury by using nylon filters as an alternative to Ficoll gradient purification and improving the outcome of islet isolation in a large animal model.

引用

页码：452 / 467

页数：16

共 43 条

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