GLUCOSE-OXIDASE OVERPRODUCING MUTANTS OF PENICILLIUM VARIABILE (P16)

被引：23
|
作者：
PETRUCCIOLI, M
PICCIONI, P
FEDERICI, F
POLSINELLI, M
机构：
[1] UNIV BASILICATA,DIPARTIMENTO BIOL DIFESA & BIOTECNOL AGROFORESTAL,I-85100 POTENZA,ITALY
[2] UNIV TUSCIA,DIPARTIMENTO AGROBIOL & AGROCHIM,I-01100 VITERBO,ITALY
[3] UNIV FLORENCE,DIPARTIMENTO BIOL ANIM & GENET,I-50125 FLORENCE,ITALY
来源：
FEMS MICROBIOLOGY LETTERS | 1995年 / 128卷 / 02期
关键词：
PENICILLIUM VARIABILE; UV MUTAGENESIS; GLUCOSE OXIDASE PRODUCTION;
D O I：
10.1016/0378-1097(95)00091-I
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Conidia of Penicillium variabile P16 were subjected to mutagenesis and selection for glucose oxidase production on media containing o-dianisidine. Studies of the relationship between dose of UV irradiation and conidial survival and frequency of mutation showed that the best frequency of positive mutation (17%) was obtained in correspondence to a conidial survival of 52%. Out of 54 overproducing mutants tested in shaken flasks, M-80.10 showed the highest level of glucose oxidase activity (127% higher than the wild-type). M-80.10 mutant, transferred every 15 days to fresh medium and tested monthly for 8 months, appeared stable. The time course of growth and enzyme production by the mutant M-80.10 showed an increase of the glucose oxidase activity in the culture medium up to 19 U ml(-1) after 96 h of fermentation.
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页码：107 / 111
页数：5
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