MicroRNA-7 enhances cytotoxicity induced by gefitinib in non-small cell lung cancer via inhibiting the EGFR and IGF1R signalling pathways

被引：23
|
作者：
Zhao, Jun-gang [1 ]
Men, Wan-fu [2 ]
Tang, Jun [2 ]
机构：
[1] China Med Univ, Shengjing Hosp, 324 Hosp Rd, Shenyang 110004, Liaoning, Peoples R China
[2] China Med Univ, Shengjing Hosp, Dept Thorac Surg, Shenyang, Liaoning, Peoples R China
来源：
WSPOLCZESNA ONKOLOGIA-CONTEMPORARY ONCOLOGY | 2015年 / 19卷 / 03期
关键词：
non-small-cell lung carcinoma; gefitinib; miR-7; epidermal growth factor receptor;
D O I：
10.5114/wo.2015.52655
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Gefitinib is a tyrosine kinase inhibitor that has been used for the treatment of non-small-cell lung carcinoma (NSCLC). The ability of miR-7 to enhance gefitinib-induced cytotoxicity in NSCLC cells was evaluated in this study. We found that miR-7 significantly decreased the IC50 of gefitinib and inhibited cell growth. G0/G1 cell cycle arrest and cell apoptosis were increased after the treatment of gefitinib coupled with miR-7 transfection. In addition, levels of Raf1, IGF1R, and PI3K and phosphorylation levels of Akt and ERK were also significantly decreased. Our results suggest that miR-7 may provide a novel therapeutic target for the treatment of NSCLCs.
引用
收藏
页码：201 / 206
页数：6
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