SENSITIVE AND SIMPLE BIOASSAY FOR HUMAN TUMOR-NECROSIS-FACTOR-ALPHA

被引：0
|
作者：
BLAGOSKLONNY, MV
NECKERS, LM
机构：
来源：
EUROPEAN CYTOKINE NETWORK | 1993年 / 4卷 / 04期
关键词：
TNF-ALPHA; BIOASSAY; U937; CELLS;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple, sensitive, and specific in vitro bioassay is described for human tumour necrosis factor alpha (TNF-alpha). The system employs the U937 cell line incubated with 10 ng/ml actinomycin D for maximum sensitivity. This method allows the detection of less than 0.6 pg/ml of TNF-alpha and the log dose-response curve is linear at concentrations 0.6-250 pg/ml TNF-alpha. Highly sensitive subclones of U937 were obtained which were capable of detecting as little as 0.1 pg/ml TNF-alpha. Finally, this bioassay demonstrates specificity by discriminating between TNF-alpha and TNF-beta.
引用
收藏
页码：279 / 283
页数：5
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