Processing challenging forensic casework samples with new protocols for the QIAsymphony SP/AS

被引：0
|
作者：
Scherer, M. [1 ]
Alsdorf, B. [1 ]
Breitbach, M. [1 ]
Matthaei, K. [1 ]
Schnur, A. [1 ]
Scholle, N. [1 ]
Bochmann, L. [1 ]
Prochnow, A. [1 ]
Engel, H. [1 ]
机构：
[1] QIAGEN GmbH, Hilden, Germany
来源：
FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES | 2013年 / 4卷 / 01期
关键词：
Forensic casework; DNA extraction; STR PCR setup; Low-volume elution; Oil overlay; Quantification data; QIAsymphony;
D O I：
10.1016/j.fsigss.2013.10.179
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Novel extraction protocols for the QIAsymphony SP improve DNA yields from forensic casework samples. The Investigator Advanced protocol has a longer heating step that enhances DNA binding to magnetic particles, while the High Efficiency protocol reduces the loss of purified DNA from low elution volumes. In addition, the latest version of the QIAsymphony AS software enables STR PCR setup normalization based on imported quantification data. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：E352 / E353
页数：2
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