In vitro multiplication of Aspidosperma ramiflorum Muell. Arg. (Apocynaceae)

被引：6
|
作者：
Hubner, Heltion Ivan [1 ]
da Silva, Luiz Vieira [1 ]
Capatti, Idivaldo [1 ]
Fumagali, Elisangela [2 ]
de Souto, Eliezer Rodrigues [3 ]
Correia Goncalves, Regina Aparecida [2 ]
Braz de Oliveira, Arildo Jose [2 ]
机构：
[1] Univ Estadual Oeste Parana, Programa Posgrad Biotecnol, Cascavel, Parana, Brazil
[2] Univ Estadual Maringa, Dept Farm & Farmacol, Ave Colombo 5790, BR-87020900 Maringa, Parana, Brazil
[3] Univ Estadual Maringa, Dept Agron, Maringa, Parana, Brazil
来源：
ACTA SCIENTIARUM-HEALTH SCIENCES | 2007年 / 29卷 / 01期
关键词：
micropropagation; Aspidosperma ramiflorum; Apocynaceae; tissue culture;
D O I：
10.4025/actascihealthsci.v29i1.108
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
The present study described a simple and promissory method for in vitro multiplication of Aspidosperma ramiflorum, a species found in the South of Brazil and seriously extinction menaced. The method was used for medicinal proposes and as a source of compounds to develop new synthetic drugs. The objective of this work was to establish an in vitro multiplication protocol of Aspidosperma ramiflorum (guatambu), from apical segments of juvenile material of plantlets obtained from seeds. The in vitro multiplication evaluation was done in WPM medium, supplemented with variable concentrations of Naphthalene acetic acid (NAA) and 6-Benzyl aminopurine (6-BAP). The multiplication of A. ramiflorum was positively influenced mainly in the combinations when 6-BAP concentrations were relatively higher than NAA. In these concentrations multiple shoots were induced.
引用
收藏
页码：63 / 66
页数：4
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