GENERAL-METHOD FOR RAPID SYNTHESIS OF MULTICOMPONENT PEPTIDE MIXTURES

被引：999

作者：

FURKA, A

SEBESTYEN, F

ASGEDOM, M

DIBO, G

机构：

[1] Department of Organic Chemistry, Eötvös Loránd university, Budapest

来源：

INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH | 1991年 / 37卷 / 06期

关键词：

PEPTIDE MIXTURES; SYNTHETIC; PEPTIDE SYNTHESIS; NEW METHOD; SOLID PHASE; PEPTIDES; ELECTROPHORETIC IDENTIFICATION; HPLC SEPARATION;

D O I：

10.1111/j.1399-3011.1991.tb00765.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is suggested for the synthesis of multicomponent peptide mixtures. The method is a solid phase synthesis modified in order to give a closely equimolar mixture of peptides with predetermined sequences. The main point of modification is that before every coupling cycle the resin is divided into equal parts and each portion is coupled with a different amino acid. Then the portions are mixed and before the next coupling cycle the resin is again distributed into equal portions. The method is illustrated by the synthesis of a mixture of 27 tetrapeptides and that of 180 pentapeptides.

引用

页码：487 / 493

页数：7