THE FOCUSING POSITIONS OF POLYPEPTIDES IN IMMOBILIZED PH GRADIENTS CAN BE PREDICTED FROM THEIR AMINO-ACID-SEQUENCES

被引：833
|
作者：
BJELLQVIST, B
HUGHES, GJ
PASQUALI, C
PAQUET, N
RAVIER, F
SANCHEZ, JC
FRUTIGER, S
HOCHSTRASSER, D
机构：
[1] UNIV GENEVA,MED CTR,DEPT MED,CH-1211 GENEVA 4,SWITZERLAND
[2] UNIV GENEVA,MED CTR,DEPT BIOCHEM,CH-1211 GENEVA 4,SWITZERLAND
来源：
ELECTROPHORESIS | 1993年 / 14卷 / 10期
关键词：
D O I：
10.1002/elps.11501401163
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The focusing Positions in narrow range immobilized pH gradients of 29 polypeptides of known amino acid sequence were determined under denaturing conditions. The isoelectric points of the proteins calculated from their amino acid sequences matched with good accuracy the experimentally determined pI values. We show the advantages of being able to predict the position of a protein of known structure within a two-dimensional gel.
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页码：1023 / 1031
页数：9
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