DIRECT RESOLUTION OF EPOXYEICOSATRIENOIC ACID ENANTIOMERS BY CHIRAL-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：14
|
作者：
ZHANG, JY [1 ]
BLAIR, IA [1 ]
机构：
[1] VANDERBILT UNIV,DEPT PHARMACOL,NASHVILLE,TN 37232
来源：
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 657卷 / 01期
关键词：
D O I：
10.1016/0378-4347(94)80065-0
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A chromatographic method was developed to separate enantiomers of four regioisomeric epoxyeicosatrienoic acids (EETs), which are cytochrome P-450 monooxygenase products of arachidonic acid. Enantiomers of individual epoxyeicosatrienoic acids were separated by chiral-phase HPLC using Chiralcel OD columns. In contrast to the pre-derivatization of EETs, the method is simple and convenient. It can be readily used in analytical studies and in preparative applications. The method can also be applied to the separation of methyl ester derivatives of epoxyeicosatrienoic acid enantiomers.
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页码：23 / 29
页数：7
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